Study on change rule of chemical composition and anti-inflammatory activity of Sinomenii Caulis-Paeoniae Radix Alba compatibility in different compatibility ratios
Objective To analyze the correlation between the content variation of characteristic components in different ratios of Qingfengteng (Sinomenii Caulis,SC) to Baishao (Paeoniae Radix Alba,PRA) and their anti-inflammatory effects. Methods HPLC was used to establish the fingerprint and characteristic component quantification methods for different ratios of the SC-PRA (1∶1,1∶2,1∶3,2:1,2:3,3∶1,3∶2),to analyze the differences and correlations of components among the groups. Lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model was established. The expression levels of inflammatory factors NO,interleukin-1β (IL-1β),and tumor necrosis factor-α (TNF-α) were measured by the Griess method and ELISA to investigate the anti-inflammatory effects of the SC-PRA pair at different ratios. Principal component analysis (PCA) was applied to integrate the characteristic component content with the expression levels of inflammatory factors,to comprehensively evaluate the optimal ratio of the herbal pair from chemical and pharmacological perspectives. Results A total of 20 common peaks were determined in the SC-PRA pair of seven ratios,with seven components identified,namely gallic acid (peak 1),sinomenine (peak 7),catechin (peak 8),magnoflorine (peak 12),paeoniflorin (peak 13),1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (peak 17),and benzoyl paeoniflorin (peak 20). When the ratio of SC-PRA was 1∶2 or 1∶3,the content of sinomenine and 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose was the highest;when the ratio was 3∶1 or 3∶2,the content of magnoflorine,gallic acid,benzoyl paeoniflorin,and paeoniflorin was the highest. Cell experiments indicate that the combination of SC-PRA at ratios of 3∶2 and 1∶3 exhibits favorable anti-inflammatory activity. PCA analysis found that the comprehensive evaluation of the anti-inflammatory effect of SC-PRA was best at a ratio of 3∶2. Conclusion The research method is simple and feasible,revealing the correlation between the content variation of characteristic components and the anti-inflammatory effects of SC-PRA at different ratios through chemical component analysis and in vitro activity evaluation,laying a foundation for further quantitative-effect correlation analysis of the herbal pair,and providing a reference for determining the appropriate dosage in clinical practice.
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