Quality value transfer of material benchmarks of classical prescription Yiyi Fuzi Baijiang Powder
Objective The HPLC fingerprints of the benchmark samples of the classic prescription Yiyi Fuzi Baijiang Powder (薏苡附子败酱散,YFBP) was established,and the contents of its index components (protocatechuic acid,chlorogenic acid,caffeic acid,isochlorogenic acid A,isochlorogenic acid C,benzoylmesaconine and linoleic acid) were determined to study the quantitative value transfer pattern of YFBP and to evaluate its quality by combining with chemical pattern recognition to assess its quality. Methods By HPLC fingerprinting of 15 batches of YFBP benchmark samples,the common peaks and extraction yield range were identified,and the marker components were quantitatively determined. Simultaneously,hierarchical cluster analysis (HCA),principal component analysis (PCA),and orthogonal partial least squares-discriminant analysis (OPLS-DA) were employed to identify the key chemical components influencing the quality of YFBP. Results In 15 batches of YFBP benchmark samples,the HPLC fingerprint similarity was greater than 0.817. A total of 30 common peaks were identified,and eight common peaks were recognised,with specific attributions as follows:Peak 30 (linoleic acid) was specific to Yiyiren (Coicis Semen). Peaks 2 (salsolinol),9,and 22 (benzoylmesaconine) were specific to Fuzi (Aconiti Lateralis Radix Praeparata). Peaks 3 (protocatechuic acid),4—6,7 (chlorogenic acid),8,10 (caffeic acid),11,13,18,19,20 (isochlorogenic acid A),21 (isochlorogenic acid C),23—26 were specific to Baijiangcao (Patriniae Herba). Peaks 1,12,14—17,27—29 were common to both Coicis Semen and Patriniae Herba. The extraction rate of the 15 batches ranged from 20.06％ to 33.22％. The mass fraction of protocatechuic acid,chlorogenic acid,caffeic acid,isochlorogenic acid A,isochlorogenic acid C,benzoylmesaconine and linoleic acid were 0.012—0.076,0.025—0.308,0.011—0.047,0.011—0.049,0.014—0.044,0.031—0.111,and 0.031—0.067 mg/g. Transfer rate were 5.05％—30.86％,5.24％—21.31％,3.57％—43.22％,4.59％—18.69％,6.97％—23.13％,9.68％—62.32％,and 6.60％—19.95％. The chemometric analysis classified the 15 batches of samples into two groups,and OPLS-DA identified 14 common peaks responsible for quality differences. Among these,three peaks have been identified:peak 3 (protocatechuic acid),peak 10 (caffeic acid),and peak 20 (isochlorogenic acid A). Conclusion Based on chemical pattern recognition,an HPLC fingerprint and a multi-index component content determination method were established for the YFBP benchmark samples of a classical prescription. The process of quantitative transfer was analyzed,providing a scientific reference for quality control and the development of compound preparations based on YFBP standard samples.
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