Development of SNP markers and identification of medicinal cultivars of Paeonia lactiflora
Objective Based on reduced-representation genome,we developed a single nucleotide polymorphism (SNP) molecular marker method for the accurate identification of medicinal cultivars of Paeonia lactiflora. Methods SNP markers were developed for 13 medicinal cultivars of P. lactiflora from Zhejiang,Anhui,Sichuan and Hunan provinces based on specific-locus smplified fragment sequencing (SLAF-seq) technology. Candidate markers were screened by multiple indicators such as heterozygosity rate,deletion rate and polymorphism,and further evaluated by genetic diversity,population structure and principal component analysis. Finally,fingerprinting was performed using the simplest SNP combination that could distinguish the medicinal cultivars of P. lactiflora. Results A total of 3703329 SLAF labels were obtained by SLAF-seq sequencing,including 60749 polymorphic labels,and 412873 population SNP markers were obtained. Finally,a total of 47 core SNP marker sites selected showed high polymorphism,and the SNP fingerprint drawn by this method could accurately distinguish medicinal cultivars of P. lactiflora. Conclusion A method of SNP marker development and fingerprint mapping for medicinal cultivars of P. lactiflora based on reduced-representation genome was established,which could provide reference for variety identification of P. lactiflora and clinical application of Cynanchum otophyllum.
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