Identification,adaptive evolution and molecular dynamics simulation of PsbP gene of Eleutherococcus senticosus
Objective To screen the EsPsbP gene in Eleutherococcus senticosus,analyse its evolutionary characteristics,study the binding mode of EsPsbP protein and PsbQ protein,and explore its expression under different light. Methods The EsPsbP genes were screened and obtained based on the genomic data of E. senticosus and were subjected to bioinformatics,adaptive evolutionary analyses and molecular dynamics simulations,and the expression of EsPsbP under different light conditions was investigated based on the results of transcriptome sequencing. Results A total of 16 EsPsbP genes were screened and classified into nine groups,A—I. A total of 21 covariate gene pairs were obtained within E. senticosus and between E. senticosus and Aralia elata,and the Ka/Ks of the gene pairs were all less than 1. There was no positively selected site in EsPsbP gene,and a large number of light-responsive,hormone-responsive,and drought-responsive elements existed in the promoter of EsPsbP gene. Molecular dynamics simulations revealed that PsbQ proteins preferentially bound to EsPsbP proteins in group A. Transcriptomic data showed that nine EsPsbPs were differentially expressed after different light quality irradiation. Conclusion A total of 16 EsPsbP genes of E. senticosus have been subjected to predominantly pure selection during evolution,which has helped to maintain gene stability. The divergence of structural features of the genes may lead to differences in the binding capacity of EsPsbP and PsbQ proteins and the response to light quality changes,and these characteristics may be related to the physiological adaptations of E. senticosus and the formation of medicinal herb quality,which lays the foundation for further research on its function.
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