狼毒大戟中的酚酸化合物及α-葡萄糖苷酶抑制活性研究
Phenolic compounds from Euphorbia fischeriana and their α-glucosidase inhibitory activity
张佳 1王梦雅 1李周子祺 1戴秦 1陈意淋 1赫军 2马立威 3张维库 2续洁琨1
作者信息
- 1. 北京中医药大学中药学院&生命科学学院,北京 102488
- 2. 中日友好医院临床医学研究所&药学部,北京 100029
- 3. 齐齐哈尔医学院医药科学研究院,黑龙江 齐齐哈尔 161006
- 折叠
摘要
目的 对狼毒大戟Euphorbia fischeriana中的酚酸类化学成分进行分离和结构鉴定,并评价化合物对α-葡萄糖苷酶的抑制活性.方法 采用多种色谱技术进行分离纯化,通过红外、高分辨质谱及核磁共振等波谱学手段鉴定化合物结构,采用PNPG法测定α-葡萄糖苷酶抑制活性.结果 从狼毒大戟的根部分离并鉴定了 8个酚酸类化合物,分别为苯甲酸甲酯-4-O-α-L-呋喃阿拉伯糖基-(1→6)-β-D-葡萄糖苷(1)、苯乙基-6-O-α-L-呋喃阿拉伯糖基-β-D-葡萄糖苷(2)、东莨菪苷(3)、corilagin(4)、6-羟基-2-甲氧基苯乙酮-4-O-β-D-葡萄糖苷(5)、6-羟基-2-甲氧基苯乙酮-4-O-α-L-阿拉伯糖-(1→6)-β-D-葡萄糖苷(6)、6-羟基-2-甲氧基苯乙酮-4-O-β-D-木糖-(1→6)-β-D-葡萄糖苷(7)、erythroxyloside B(8).结论 化合物1为新化合物,命名为狼毒酚苷A(euphfischcoside A),化合物2～4为首次从大戟属中分离得到.化合物4和8对α-葡萄糖苷酶的半数抑制浓度(median inhibition concentration,IC50)值分别为(7.44±3.95)µmol/L 和(134.40±30.27)μmol/L.
Abstract
Objective To investigate the chemical constituents of the roots of Euphorbia fischeriana and evaluate their α-glucosidase inhibitory activity.Methods A variety of chromatographic techniques were applied for the chemical separation and purification.Their structures were elucidated by a combination of IR,HR-ESI-MS and NMR spectra.PNPG method was performed to evaluate theα-glucosidase inhibitory activity.Results Eight phenolic compounds were isolated from the roots of E.fischeriana.Their structures were identified as methyl benzoate-4-O-α-L-arabinofuranosyl-(1→6)-β-D-glucopyranoside(1),phenethyl 6-O-α-L-arabinofuranosyl-β-D-glucopyranoside(2),scopoletin(3),corilagin(4),6-hydroxy-2-methoxyacetophenone-4-O-β-D-glucopyranoside(5),6-hydroxy-2-methoxyacetophenone-4-O-α-L-arabinofuranosyl-(1→6)-β-D-glucopyranoside(6),6-hydroxy-2-methoxyacetophenone-4-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside(7),erythroxyloside B(8).Conclusions Compound 1 is a new compound and named as euphfischcoside A,compounds 2-4 were isolated from E.fischeriana for the first time.Compounds 4 and 8 exhibit the considerable inhibition on α-glucosidase with the IC50 values of(7.44±3.95)μmol/L and(134.40±30.27)μmol/L,respectively.
关键词
狼毒大戟/酚酸化合物/糖苷/α-葡萄糖苷酶/狼毒酚苷A/苯乙基-6-O-α-L-呋喃阿拉伯糖基-β-D-葡萄糖苷/东莨菪苷Key words
Euphorbia fischeriana Steud./phenolic acid/glucosides/α-glucosidase/euphfischcoside A/phenethyl 6-O-α-L-arabino-furanosyl-β-D-glucopyranoside/scopoletin引用本文复制引用
出版年
2025
NETL
NSTL
万方数据