丹参内生真菌Alternaria malvae中聚酮类成分研究
Polyketones in endophytic fungus Alternaria malvae from Salvia miltiorrhiza
王思蕊 1孙冉 1张宏燕 1章玲 1孙云鹏 2郁阳 2刘劲松 2王国凯2
作者信息
- 1. 安徽中医药大学药学院中药功效物质重点实验室,安徽 合肥 230012
- 2. 安徽中医药大学药学院中药功效物质重点实验室,安徽 合肥 230012;安徽省活性天然产物重点实验室,安徽 合肥 230012
- 折叠
摘要
目的 研究丹参Salvia miltiorrhiza内生真菌细链格孢Alternaria malvae的次生代谢产物及生物活性.方法 采用正相柱色谱、MPLC-ODS、MCI、Sephadex LH-20,结合制备型高效液相等色谱手段进行分离纯化,利用核磁共振和质谱技术对单体化合物进行鉴定,通过检测脂多糖(lipopolysaccharide,LPS)诱导小鼠巨噬细胞(RAW264.7)一氧化氮(NO)的释放,评价化合物的抗炎活性.结果 从细链格孢的大米发酵产物中分离得到7个化合物,分别鉴定为3-甲氧基细链格孢内酯B(1)、4'-表细链格孢烯(2)、(+)-(4'R,5'S,7'R)-细链格孢烯(3)、4'-表-二氢细链格孢烯A(4)、细链格孢三醇5-甲醚(5)、细链格孢三醇(6)和细链格甲醚孢醇(7).化合物5和7表现出抗炎活性,半数抑制浓度(median inhibition concentration,IC50)值分别为(14.80±0.67)μmol/L和(27.76±2.75)μmol/L.结论 化合物1为聚酮类新化合物,命名为3-甲氧基细链格孢内酯B;化合物5有显著抗炎活性.
Abstract
Objective To study the secondary metabolites and its biological activities of the endophyties Alternaria malvae derived from the roots of Salvia miltiorrhiza.Methods Preparative high-performance liquid chromatography,along with additional chromatographic techniques including normal-phase column chromatography,MPLC-ODS,MCI,and Sephadex LH-20,was employed to isolate and purify the secondary metabolites.NMR and mass spectrometry were then used to characterize the individual compounds.Results A total of seven compounds were isolated from the rice fermentation of A.malvae and identified as 3-methoxyalternolide B(1),4'-epialtenuene(2),(+)-(4'R,5'S,7'R)-altenuene(3),4'-epi-dihydroaltenuene A(4),alternariol 5-methyl ether(5),alternariol(6)and altenuisol(7).Compound 1 was a new compound,Compounds 5 and 7 exhibited anti-inflammatory activity with IC50 values of(14.8+0.67)μmol/L and(27.76+2.75)μmol/L,respectively.Conclusion Compound 1 is a new polyketone compound named 3-methoxyalternolide B.Compound 5 has significant anti-inflammatory activity.
关键词
丹参/内生真菌/聚酮/细链格孢/3-甲氧基细链格孢内酯B/细链格孢三醇5-甲醚/抗炎活性Key words
Salvia miltiorrhiza Bunge/endophytic fungi/polyketones/Alternaria malvae Roum.& Letell./3-methoxyalternolide B/alternariol 5-methyl ether/anti-inflammatory activity引用本文复制引用
出版年
2025
NETL
NSTL
万方数据