首页|丹参内生真菌Alternaria malvae中聚酮类成分研究

丹参内生真菌Alternaria malvae中聚酮类成分研究

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目的 研究丹参Salvia miltiorrhiza内生真菌细链格孢Alternaria malvae的次生代谢产物及生物活性.方法 采用正相柱色谱、MPLC-ODS、MCI、Sephadex LH-20,结合制备型高效液相等色谱手段进行分离纯化,利用核磁共振和质谱技术对单体化合物进行鉴定,通过检测脂多糖(lipopolysaccharide,LPS)诱导小鼠巨噬细胞(RAW264.7)一氧化氮(NO)的释放,评价化合物的抗炎活性.结果 从细链格孢的大米发酵产物中分离得到7个化合物,分别鉴定为3-甲氧基细链格孢内酯B(1)、4'-表细链格孢烯(2)、(+)-(4'R,5'S,7'R)-细链格孢烯(3)、4'-表-二氢细链格孢烯A(4)、细链格孢三醇5-甲醚(5)、细链格孢三醇(6)和细链格甲醚孢醇(7).化合物5和7表现出抗炎活性,半数抑制浓度(median inhibition concentration,IC50)值分别为(14.80±0.67)μmol/L和(27.76±2.75)μmol/L.结论 化合物1为聚酮类新化合物,命名为3-甲氧基细链格孢内酯B;化合物5有显著抗炎活性.
Polyketones in endophytic fungus Alternaria malvae from Salvia miltiorrhiza
Objective To study the secondary metabolites and its biological activities of the endophyties Alternaria malvae derived from the roots of Salvia miltiorrhiza.Methods Preparative high-performance liquid chromatography,along with additional chromatographic techniques including normal-phase column chromatography,MPLC-ODS,MCI,and Sephadex LH-20,was employed to isolate and purify the secondary metabolites.NMR and mass spectrometry were then used to characterize the individual compounds.Results A total of seven compounds were isolated from the rice fermentation of A.malvae and identified as 3-methoxyalternolide B(1),4'-epialtenuene(2),(+)-(4'R,5'S,7'R)-altenuene(3),4'-epi-dihydroaltenuene A(4),alternariol 5-methyl ether(5),alternariol(6)and altenuisol(7).Compound 1 was a new compound,Compounds 5 and 7 exhibited anti-inflammatory activity with IC50 values of(14.8+0.67)μmol/L and(27.76+2.75)μmol/L,respectively.Conclusion Compound 1 is a new polyketone compound named 3-methoxyalternolide B.Compound 5 has significant anti-inflammatory activity.

Salvia miltiorrhiza Bungeendophytic fungipolyketonesAlternaria malvae Roum.& Letell.3-methoxyalternolide Balternariol 5-methyl etheranti-inflammatory activity

王思蕊、孙冉、张宏燕、章玲、孙云鹏、郁阳、刘劲松、王国凯

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安徽中医药大学药学院中药功效物质重点实验室,安徽 合肥 230012

安徽省活性天然产物重点实验室,安徽 合肥 230012

丹参 内生真菌 聚酮 细链格孢 3-甲氧基细链格孢内酯B 细链格孢三醇5-甲醚 抗炎活性

2025

中草药
天津药物研究院,中国药学会

中草药

北大核心
影响因子:1.632
ISSN:0253-2670
年,卷(期):2025.56(2)