A new aporphine and amide alkaloids from Artabotrys hexapetalus and their cytotoxic activities
Objective To investigate the chemical constituents and cytotoxic activities of Artabotrys hexapetalus.Methods The 95％ethanol extracts of the stems and roods of A.hexapetalus were purified on the basis of several chromatographic methods.The structural identification of isolated compounds were based on NMR,HRESIMS and ECD calculation.The cytotoxic activity against three types of tumor cells(HepG2,A549,HCT116)were tested by the CCK-8 methods.Results One undescribed aporphine alkaloid and seven previously reported amide alkaloids were isolated from the 95％ethanol extracts and elucidated as(E)-(6aS,7R)-7-hydroxy-3-methoxy-N-acrylaldehyde-1,2-methylenedioxy oxoaporphine alkaloid(1),trans-N-p-coumaroyltyramine(2),N-trans-3-hydroxy-4-methoxycinnamoyltyramine(3),N-trans-feruloyl-tyramine(4),(E)-3-(4-hydroxy-3-methoxyphenyl)-N-phenethyl-acrylamide(5),northalifoline(6),tribulusamide A(7),and cannabisin F(8).Compounds 7 and 8 showed significantly cytotoxic activity against HepG2 cells with IC50 values of(30.52±0.93)μmol/L and(16.26±1.97)μmol/L,respectively.Conclusions Compound 1 was an unreported compound named hexapetalusine E,and compounds 3-8 were isolated for the first time from the genus Artabotrys.Compounds 7 and 8 showed significant cytotoxicity against HepG2 cells.
Artabotrys hexapetalus(L.f.)Bhandariaporphine alkaloidamide alkaloidcytotoxicityhexapetalusine Ecannabisin F
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