Chemical constituents and anti-inflammatory activity of Atractylodes macrocephala
Objective To study the chemical constituents from the stems of Atractylodes macrocephala.Methods The chemical constituents ofA.macrocephala were systematically isolated and purified by silica gel,sephadex LH-20 gel column chromatography,semi-preparation HPLC and recrystallization method,and the structures of the compounds were identified by spectral analysis and physicochemical properties.Meanwhile,anti-inflammatory activity of the compounds was evaluated by LPS-induced RAW264.7 macrophage model.Results A total of 42 compounds were identified as guaiannediol(1),1-patchoulene-4α,7α-diol(2),7β-hydroxy-7-epi-α-eudesmol(3),3-eudesmene-1β,7,11-triol(4),cespilamide C(5),linoleic acid(6),oleic acid(7),linolenic acid(8),(S)-coriolic acid(9),(8E,10E)-7-oxo-8,10-heptadecadienoic acid(10),(10E,12E)-9-oxooctadeca-10,12-dienoic acid(11),(S)-coriolic acid methyl easter(12),11-hydroxyeicosanoic acid(13),benzene 1,2,4-triol(14),2-(4-methoxyphenyl)acetic acid(15),methyl 4-yhdroxy-3-methoxyphenylacetate(16),4-ethylcatechol(17),2-bromo-1,4-hydroquione(18),4-methylbenzene-1,2-diol(19),4-nitrophenol(20),m-hydroxybenzoic acid(21),protocatechoic acid(22),vanillic acid(23),4-hydroxy-3-methoxypropiphenone(24),2,4-dihydroxy-3-methylben zaldehyde(25),salicylaldehyde(26),3-hydroxybenzaldehyde(27),magnolol(28),fraxidin(29),cleomiscosin(30),atractylochromene(31),(+)-kazinol B(32),schaftoslde(33),genistein-7,4'-O-β-D-diglucoside(34),rutinum(35),kaempferol 3-O-β-D-sophoroside-7-O-α-L-rhamnoside(36),kaempferol 3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranosyl-(1 → 2)-β-D-glucopyranoside(37),kaempferol 3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranosyl-(1→ 2)-β-D-glucopyranoside(38),(R)-2-propylhexyl-2H-1,2,3-triazole-4-carboxylate(39),1-acetyl-β-carboxylate(40),2-beno-thiazolol(41),and magnoflorine(42)from the stems of A.macrocephala,respectively.Conclusion Compound 1 is a new natural product and compounds 1-42 were isolated from genus of Atractylodes for the first time.Compound 13 inhibited the release of NO in RAW264.7 cells induced by LPS with IC50 value of(8.21±0.17)μmol/L.
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