摘要
目的 研究佛手Citrus medica var.sarcodactylis干燥果实的化学成分.方法 应用正相硅胶、Sephadex LH-20凝胶柱色谱、ODS以及半制备型HPLC等色谱技术进行系统分离纯化,并且通过核磁共振波谱、质谱等技术鉴定单体化合物的结构.结果 从佛手95％乙醇提取物中共分离得到26个化合物,分别鉴定为大黄酚(1)、大黄素甲醚(2)、大黄素(3)、芦荟大黄素(4)、卡枯醇(5)、2-甲氧基-4,5-亚甲二氧基苯丙酮(6)、methoxylatifolone(7)、和厚朴酚(8)、牛蒡子苷元(9)、罗汉松树脂酚(10)、表松脂酚(11)、lappaol F(12)、arctium B(13)、isolappaol A(14)、lappaol A(15)、3-(1,3-benzodioxol-5-yl)-1,2-propanediol(16)、lappaol B(17)、丁香脂素(18)、吴茱萸次碱(19)、墙草碱(20)、asaroidoxazine B(21)、蔓荆子黄素(22)、2-(3,4-dimethoxyphenyl)-3,5,8-trihydroxy-7-methoxy-4H-1-benzopyran-4-one(23)、2-(3,4-dimethoxyphenyl)-5,6,7-trihydroxy-3-methoxy-4H-1-benzopyran-4-one(24)、(22 E,24R)-5α,8α,-过氧麦角甾-6,9,22-三烯-3β-醇(25)和过氧麦角甾醇(26).结论 所有化合物均为首次从佛手中分离得到,为佛手的开发利用提供一定的物质基础.
Abstract
Objective To study the chemical constituents from the fruits of Citrus medica.var.sarcodactylis.Methods The chemical constituents were isolated and purified by silica gel,Sephadex LH-20 gel column chromatography,ODS and semi-preparation HPLC.The structures of all isolates were determined on the basis of NMR and MS analysis.Results Atotal of 26 compounds were isolated from the 95％EtOH extract of C.medica var.sarcodactylis,which were identified as chrysophanic acid(1),physcion(2),emodin(3),aloeemodin(4),kakuol(5),2-methoxy-4,5-methylenedioxypropiophenone(6),methoxylatifolone(7),honokiol(8),arctigenin(9),matairesinol(10),epipinoresinol(11),lappaol F(12),arctium B(13),isolappaol A(14),lappaol A(15),3-(1,3-benzodioxol-5-yl)-1,2-propanediol(16),lappaol B(17),syringaresinol(18),rutaecarpine(19),pellitorine(20),asaroidoxazine B(21),casticin(22),2-(3,4-dimethoxyphenyl)-3,5,8-trihydroxy-7-methoxy-4H-1-benzopyran-4-one(23),2-(3,4-dimethoxyphenyl)-5,6,7-trihydroxy-3-methoxy-4H-1-benzopyran-4-one(24),(22E,24R)-5α,8α,-epidioxyergosta-6,9,22-triene-3β-ol(25)and ergosterol peroxide(26).Conclusion All compounds were isolated from the fruits of C.medica var.sarcodactylis for the first time.This study provides certain study basis for the utilization of C.medica.var.sarcodactylis.
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