Investigation of the effects of decoction methods and purification processes on medicinal property transfer of classic prescription Wuzhuyu Decoction based on phase transition
Objective To explore the impact of different decoction methods and purification processes on physical phase and chemical composition of Wuzhuyu Decoction(吴茱萸汤,WD),ensuring the medicinal properties of"a bowl of decoction"in the development of classic prescriptions.Methods The traditional decoction method of WD was determined through literature review and experimentation,with the decoction method recorded in Management Specifications for the Decoction Room of Traditional Chinese Medicine in Medical Institutions serving as the modem decoction method.The two types of WD were refined using aqueous extraction and alcohol precipitation,and a clarifying agent method.Differential centrifugation combined with dialysis was used to separate different phases of WD.The morphology,particle size,ζpotential,particle concentration,polysaccharide and protein concentration of the nanophase in the decoction were measured and compared.The yield of the original WD and different phases were measured,and the contents of ten compounds,including 6-O-caffeoylglucopyranose,chlorogenic acid,hyperoside,dehydroevodiamine,ginsenoside Rg1,ginsenoside Rb1,limonin,6-gingerol,evodiamine and rutecarpine,were determined by UPLC-UV.Results Spherical nanoparticles with a diameter of about 200 nm and a mesoporous surface were found in the nanophase of WD.Both purification processes reduce the number of particles in the nanophase,disrupt the structure of the particles,and decrease the content of protein and polysaccharides.The yield rate of modern decoction of WD and its freeze-dried powder of the nanophase were both significantly higher than those of the traditional decoction of WD,the content of the eight components other than evodiamine and rutaecarpine in modern decoction of WD are all significantly higher than those of the traditional decoction of WD.After treatment with the clarifying agent,the content of most components decreases or shows a decreasing trend in nanophase,while after treatment with alcohol precipitation,the content of most components increases or shows an increasing trend in nanophase.The distribution ratio of each component also differs from that in the nanophase of the original decoction.Conclusion Different decoction methods and purification processes significantly affect both the chemical composition and structural properties of the classic prescription WD,which should be considered in the research and development process of classic prescriptions and other Chinese herbal compound preparations.
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