Quality evaluation of Xiangguo Jianxiao Tablets based on HPLC fingerprint combined with chemometrics and multi-component content determination
Objective To establish an HPLC fingerprint method and a method for the determination of the multi-component content of Xiangguo Jianxiao Table(XJT).Methods The components were analyzed on an Infinity Lab Poroshell 120 SB-C18 column(250 mm × 4.6 mm,4 μm).The gradient elution was carried out with the mobile phase composed of 0.15％formic acid water and acetonitrile at the flow rate of 1.0 mL/min and the column temperature of 30 ℃.The detecting wavelength was 254 nm and injection volume was 10 μL.Based on the fingerprinting of XJT,hierarchical cluster analysis(HCA)and principal component analysis(PCA)were used to evaluate the quality of different batches of XJT,while Hotelliing's T2 and DModX methods were used to analyze the quality control range of different batches of XJT.HPLC was used to determine the contents of nine components,namely protocatechuic acid,chlorogenic acid,caffeic acid,isochlorogenic acid B,isochlorogenic acid A,hesperidin,isochlorogenic acid C,costunolide and dehydrocostus iactone in 13 batches of XJT.Results The methodological investigation of XJT fingerprints met the requirements,labelled 18 common components,identified nine components by control identification,and the similarity of 13 batches of samples was greater than 0.90.HCA and PCA showed good inter-batch consistency,and the upper control limits of Hotelliing's T2 and DModXwere 23.58 and 1.70.The contents of protocatechuic acid,chlorogenic acid,caffeic acid,isochlorogenic acid B,isochlorogenic acid A,hesperidin,isochlorogenic acid C,costunolide and dehydrocostus iactone in 13 batches of XJT ranged from 23.0-68.7,558.7-2 463.9,39.3-109.6,117.1-294.2,228.7-1 001.7,372.2-839.1,151.4-800.6,124.3-1 073.9,589.0-2 229.8 μg/g,respectively.Conclusion The HPLC fingerprinting method and multi-component content determination method were accurate and reliable,and can be used for the quality evaluation of XJT.
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