目的 采用UPLC-MS/MS法测定麦冬Ophiopogonis Radix多指标成分结合偏最小二乘法-判别分析(partial least squares-discriminant analysis，PLS-DA)、熵权-TOPSIS法对不同厂家参麦颗粒质量进行综合评价。方法 采用UPLC-MS/MS法同时测定3家厂家85批参麦颗粒中麦冬苷元-3-O-α-L-吡喃鼠李糖-β-D-吡喃葡萄糖苷、龙脑7-O-[β-D-呋喃芹菜糖基-(1→6)]-β-D-吡喃葡萄糖苷、麦冬皂苷C、麦冬皂苷D、短葶山麦冬皂苷C、山麦冬皂苷B、甲基麦冬高黄酮A、甲基麦冬高黄酮B、甲基麦冬黄烷酮A、甲基麦冬黄烷酮B、麦冬黄烷酮A和对香豆酸12个麦冬成分的含量，运用PLS-DA及熵权-TOPSIS法建立参麦颗粒中投料用麦冬饮片综合质量优劣评价方法。结果 12个成分分别在考察的质量浓度范围内线性关系良好(r＞0。998 9)，加样回收率为95。3％～105。3％，RSD值均小于2。9％，精密度、重复性、稳定性考察均符合分析要求。85批样品差异明显，甲基麦冬黄烷酮B、麦冬皂苷D、甲基麦冬高黄酮B、甲基麦冬黄烷酮A、甲基麦冬高黄酮A、麦冬苷元-3-O-α-L-吡喃鼠李糖-β-D-吡喃葡萄糖苷和麦冬皂苷C是影响参麦颗粒中麦冬质量的主要潜在标志物;熵权-TOPSIS法可用于不同厂家参麦颗粒中投料用麦冬的质量优劣的评价。结论 建立的多指标成分定量测定方法操作便捷、结果准确，结合PLS-DA及熵权-TOPSIS法可用于参麦颗粒中投料用麦冬的质量的综合评价。
Comprehensive quality evaluation of Shenmai Granules based on multi-target ingredients of Ophiopogonis Radix combined with PLS-DA and EW-TOPSIS method
Objective To comprehensively evaluate the quality of Shenmai Granules(参麦颗粒,SG)from different manufacturers by using UPLC-MS/MS method to determine the multi-indicator components of Maidong(Ophiopogonis Radix)combined with partial least squares discriminant analysis(PLS-DA)and entropy weight(EW)-TOPSIS mothod.Methods The UPLC-MS/MS method was used to simultaneously determine the contents of 12 Ophiopogon japonicus components,including ophiogenin-3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside,borneol 7-O-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside,ophiopojaponin C,ophiopojaponin D,liriope muscari baily saponins C,liriopesides B,methylophiopogonone A,methylophiopogonone B,methylophiopogonanone A,methylophiopogonanone B,ophiopogonanone A and p-coumaric acid in 85 batches of SG from three manufacturers.A comprehensive quality evaluation method for the Ophiopogon japonicus in SG was established using PLS-DA and EW-TOPSIS.Results The 12 components showed good linear relationships in their own ranges.The average recovery were 95.3％-105.3％,the RSDs were all less than 2.9％,and the precision,repeatability,and stability tests met the analytical requirements.The samples showed significant differences,methylophiopogonanone B,ophiopojaponin D,methylophiopogonone B,methylophiopogonanone A,methylophiopogonone A,ophiogenin 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside and ophiopojaponin C were the main potential markers affecting the quality.The EW-TOPSIS method can be used to evaluate the quality of O.japonicus used in different manufacturers'SG.Conclusion The established multi-index ingredients quantitative determination method is convenience and accuracy,and the combination of PLS-DA and EW-TOPSIS method can be used for the comprehensive evaluation of the quality of Ophiopogonis Radix for feeding in SG.
Shenmai GranulesUPLC-MS/MSOphiopogonis Radixpartial least squares discriminant analysisentropy weight-TOPSIS methodophiogenin-3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranosideborneol 7-O-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranosideophiopojaponin C
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