基于麦冬多指标成分定量结合偏最小二乘法-判别分析、熵权-TOPSIS法评价参麦颗粒质量
Comprehensive quality evaluation of Shenmai Granules based on multi-target ingredients of Ophiopogonis Radix combined with PLS-DA and EW-TOPSIS method
雷蓉 1杨建玲 2周亚楠 1刘亚茹 1刘永利 1马双成3
作者信息
- 1. 河北省药品医疗器械检验研究院河北省中药质量评价与标准研究重点实验室,河北石家庄 050227
- 2. 北京儿童医院保定医院,河北保定 071000
- 3. 国家药典委员会,北京 100061
- 折叠
摘要
目的 采用UPLC-MS/MS法测定麦冬Ophiopogonis Radix多指标成分结合偏最小二乘法-判别分析(partial least squares-discriminant analysis,PLS-DA)、熵权-TOPSIS法对不同厂家参麦颗粒质量进行综合评价.方法 采用UPLC-MS/MS法同时测定3家厂家85批参麦颗粒中麦冬苷元-3-O-α-L-吡喃鼠李糖-β-D-吡喃葡萄糖苷、龙脑7-O-[β-D-呋喃芹菜糖基-(1→6)]-β-D-吡喃葡萄糖苷、麦冬皂苷C、麦冬皂苷D、短葶山麦冬皂苷C、山麦冬皂苷B、甲基麦冬高黄酮A、甲基麦冬高黄酮B、甲基麦冬黄烷酮A、甲基麦冬黄烷酮B、麦冬黄烷酮A和对香豆酸12个麦冬成分的含量,运用PLS-DA及熵权-TOPSIS法建立参麦颗粒中投料用麦冬饮片综合质量优劣评价方法.结果 12个成分分别在考察的质量浓度范围内线性关系良好(r＞0.998 9),加样回收率为95.3％～105.3％,RSD值均小于2.9％,精密度、重复性、稳定性考察均符合分析要求.85批样品差异明显,甲基麦冬黄烷酮B、麦冬皂苷D、甲基麦冬高黄酮B、甲基麦冬黄烷酮A、甲基麦冬高黄酮A、麦冬苷元-3-O-α-L-吡喃鼠李糖-β-D-吡喃葡萄糖苷和麦冬皂苷C是影响参麦颗粒中麦冬质量的主要潜在标志物;熵权-TOPSIS法可用于不同厂家参麦颗粒中投料用麦冬的质量优劣的评价.结论 建立的多指标成分定量测定方法操作便捷、结果准确,结合PLS-DA及熵权-TOPSIS法可用于参麦颗粒中投料用麦冬的质量的综合评价.
Abstract
Objective To comprehensively evaluate the quality of Shenmai Granules(参麦颗粒,SG)from different manufacturers by using UPLC-MS/MS method to determine the multi-indicator components of Maidong(Ophiopogonis Radix)combined with partial least squares discriminant analysis(PLS-DA)and entropy weight(EW)-TOPSIS mothod.Methods The UPLC-MS/MS method was used to simultaneously determine the contents of 12 Ophiopogon japonicus components,including ophiogenin-3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside,borneol 7-O-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside,ophiopojaponin C,ophiopojaponin D,liriope muscari baily saponins C,liriopesides B,methylophiopogonone A,methylophiopogonone B,methylophiopogonanone A,methylophiopogonanone B,ophiopogonanone A and p-coumaric acid in 85 batches of SG from three manufacturers.A comprehensive quality evaluation method for the Ophiopogon japonicus in SG was established using PLS-DA and EW-TOPSIS.Results The 12 components showed good linear relationships in their own ranges.The average recovery were 95.3％-105.3％,the RSDs were all less than 2.9％,and the precision,repeatability,and stability tests met the analytical requirements.The samples showed significant differences,methylophiopogonanone B,ophiopojaponin D,methylophiopogonone B,methylophiopogonanone A,methylophiopogonone A,ophiogenin 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside and ophiopojaponin C were the main potential markers affecting the quality.The EW-TOPSIS method can be used to evaluate the quality of O.japonicus used in different manufacturers'SG.Conclusion The established multi-index ingredients quantitative determination method is convenience and accuracy,and the combination of PLS-DA and EW-TOPSIS method can be used for the comprehensive evaluation of the quality of Ophiopogonis Radix for feeding in SG.
关键词
参麦颗粒/UPLC-MS/MS/麦冬/偏最小二乘-判别分析/熵权-TOPSIS法/麦冬苷元-3-O-α-L-吡喃鼠李糖-β-D-吡喃葡萄糖苷/龙脑7-O-[β-D-呋喃芹菜糖基-(1→6)]-β-D-吡喃葡萄糖苷/麦冬皂苷C/麦冬皂苷D/短葶山麦冬皂苷C/山麦冬皂苷B/甲基麦冬高黄酮A/甲基麦冬高黄酮B/甲基麦冬黄烷酮A/甲基麦冬黄烷酮B/麦冬黄烷酮A/对香豆酸Key words
Shenmai Granules/UPLC-MS/MS/Ophiopogonis Radix/partial least squares discriminant analysis/entropy weight-TOPSIS method/ophiogenin-3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside/borneol 7-O-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside/ophiopojaponin C引用本文复制引用
出版年
2025
NETL
NSTL
万方数据