Mechanism of Aquilariae Lignum Resinatum sesquiterpene ZH-13 in protecting neuronal injury induced by endoplasmic reticulum stress
Objective To explore the improvement effect and mechanism of action of the active component sesquiterpene ZH-13 on endoplasmic reticulum(ER)stress-induced neuronal injury based on the traditional sedative effect of Chenxiang(Aquilariae Lignum Resinatum).Methods The ER stress inducer tunicamycin(Tm)was used to trigger ER stress in PC12 cells,and the effects of ZH-13 on cell viability,apoptosis,and expression of ER stress-related proteins were examined.Results The results showed that Tm reduced PC12 cell in a dose-dependent manner(P＜0.01),and ZH-13 significantly increased cell viability(P＜0.01)and ameliorated ER stress.By analyzing the proteins related to protein kinase RNA-like endoplasmic reticulum kinase(PERK),inositol-requiring enzyme 1(IRE1),and activating transcription factor 6(ATF6),it was confirmed that ZH-13 significantly inhibited the overactivation of the PERK pathway in ER stress(P＜0.05),with no obvious effect on the IRE1 and ATF6 pathways.Conclusion ZH-13 improves ER stress-mediated damage in PC12 cells by regulating the PERK pathway,demonstrating a good neuroprotective effect.
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