Screening strategies for high expression of GsSTS50 gene of Ganoderma sinense in Escherichia coli
Objective The low content of secondary metabolites of Ganoderma sinense has brought some difficulties to the development of monomers,so it is necessary to screen a method that can significantly increase the yield of secondary metabolites of G.sinense.Methods The GsSTS50 gene encoding linalool synthetase was selected based on the G.sinense gene library.The GsSTS50 gene was overexpressed in Escherichia coli by co-expression and introduction of heterologous mevalonate(MVA)pathway.The linalool content in E.coli was determined by HS-SPME-GC-MS.Results The yield of linalool and other terpenoids in E.coli was increased by about 4.9 times by optimizing the strategy of fusion enzyme.By introducing heterologous MVA pathway,the production of linalool was 49 times higher than that of the original culture.The co-expression of multiple genes at the same time into the heterologous pathway,the yield of the optimized strain was 42 times higher than that of the original strain.Conclusion The results showed that the yield of recombinant strains was significantly increased.This study provides a reference for further research on terpenoids and other terpenoids from G.sinense.
Ganoderma sinense Zhao Xu et Zhang.linaloolHS-SPME-GC-MSmevalonate pathwaybiological metabolism
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