Quality evaluation of Asteris Radix et Rhizoma from different sources based on UPLC fingerprint and multi-index component determination combined with chemometrics
Objective To establish an ultra-high performance liquid chromatography(UPLC)fingerprint and multi-component content determination method for Ziwan(Asteris Radix et Rhizoma)from different sources,and evaluate the quality of Asteris Radix et Rhizoma from different sources in combination with chemometrics methods,so as to provide technical method reference and basic data for its quality control research.Methods Phenomenex Titank C18 chromatographic column(150 mm × 2.1 mm,1.8 μm)was used,with 0.2％formic acid water(A)-acetonitrile(B)solution as mobile phase,gradient elution(0-3 min,5％-9％B;3-13 min,9％-10％B;13-15 min,10％-16％B;15-20 min,16％B;20-50 min,16％-28％B;50-54 min,28％-51％B;54-64 min,51％-100％B;64-74 min,100％B;74-76 min,100％-5％B).The flow rate was 0.3 mL/min,the column temperature was 40 ℃,the injection volume was 3 μL,and the detection wavelength was set at a fixed wavelength(0-50 min,325 nm;50-70 min,260 nm;70-76 min,203 nm).The fingerprints of Asteris Radix et Rhizoma from different sources were constructed,and the contents of chlorogenic acid,caffeic acid,ferulic acid,quercetin,kaempferol and shionone were determined by similarity analysis and chemometrics analysis.Results The fingerprints of Asteris Radix et Rhizoma were established.A total of 17 common peaks were marked.Nine chromatographic peaks were identified by the comparison method of reference substances and MS identification,which were peak 1(chlorogenic acid),peak 3(caffeic acid),peak 5(ferulic acid),peak 10(quercetin),peak 11(asterin),peak 12(kaempferol),peak 13(liquiritigenin),peak 16(stigmasterol glucoside),and peak 17(shionone).Eighteen batches of Asteris Radix et Rhizoma samples were divided into Hebei origin and Anhui origin by cluster analysis(CA).Principal component analysis(PCA)showed that there were differences among Asteris Radix et Rhizoma samples from different habitats.According to the VIP analysis under orthogonal partial least squares-discriminant analysis(OPLS-DA),peaks 11(asterin),12(kaempferol),1(chlorogenic acid),14,10(quercetin),9,13(liquiritigenin),17(shionone)may be a marker affecting the quality of Asteris Radix et Rhizoma from different habitats and different processing methods.The contents of chlorogenic acid,caffeic acid,ferulic acid,kaempferol,quercetin and shionone ranged from 5.941 6-18.745 1,0.477 2-1.046 6,0.177 4-0.265 6,1.135 8-1.720 7,0.574 9-2.755 7,148.3 408-252.1 639 μg/g.According to the methodology,each component showed a good linear relationship.The content determination results showed that sulfur fumigation of Asteris Radix et Rhizoma had a great effect on the content of shionone,and the content of shionone decreased significantly.Conclusion The quality of Asteris Radix et Rhizoma from different habitats was distinguished by the evaluation method established by quantitative analysis of multi index components combined with chemometrics,and the content differences of chemical components in Asteris Radix et Rhizoma from different processing methods were evaluated,which could provide scientific basis and reference for the quality evaluation and control of Asteris Radix et Rhizoma.
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