Detection of gene mutations and clinical pathological characteristics in 442 cases of papillary thyroid carcinoma by Next-Generation Sequencing
Objective To investigate the genetic changes in papillary thyroid carcinoma(PTC)and their relationship with clinical pathological features.Methods Next-Generation Sequencing was used to conduct Genetic testing in 442 patients with PTC,and the clinicopathological data of the patients were collected at the same time.Results 1.Among the 442 PTC patients,423 patients detected gene mutations,including BRAF(385 cases),RET(24 cases),KRAS(5 cases),NTRK3(3 cases)and NTRK1(3 cases).2.BRAF mutations were only associated with the maximum diameter of the tumor(P=0.006).3.The abundance of V600E mutations was correlated with gender,maximum tumor diameter,single/bilateral tumor,extraglandular invasion,lymph node metastasis,and histological subtypes(all P＜0.05).4.The abundance of different mutations in V600E is correlated with the maximum diameter of the tumor,distribution of cancer foci,extraglandular invasion,lymph node metastasis,and histological subtypes(all P＜0.05).Conclusion The application of NGS can clarify the unique characteristics of PTC driver gene mutations,and BRAF V600E mutation is associated with multiple high-risk clinical pathological features.
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