Relationship between KDM6A protein expression and gene mutation and mismatch repair in patients with colorectal cancer
Objective To investigate the relationship between KDM6A protein expression and gene mutation and mismatch repair in patients with colorectal cancer(CRC).Methods The 111 patients with surgically resected CRC cases were collected.KDM6A protein and MMR proteins(MLH1,MSH2,MSH6,and PMS2)expression was determined by immunohistochemistry,and KRAS/NRAS/BRAF/PIK3CA gene mutations were identified by the amplification refractory mutation system(ARMS),and analyzed statistically for the relationship between KDM6A expression and gene mutation,MMR,the clinicopathological features,and prognosis,which was validated by CRC data from the TCGA database.Results KDM6A expression rate was significantly elevated in CRC patients with mutations.CRC patients with KDM6A expression were likely to develop proficient mismatch repair(pMMR).KDM6A expression was significantly higher in CRC patients with vascular invasion than in those without vascular invasion.No significant differences were identified in overall survival(OS)between the KDM6A-positive and KDM6A-negative groups in patients with CRC cancer.Conclusion KDM6A expression in CRC patients is associated with mutations in the KRAS/NRAS/BRAF/PIK3CA genes,more common in patients with pMMR,and more likely occurs in patients with vascular invasion,but not associated with the prognosis.
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