Comparison of the results using cervical tissues and exfoliated cervical cells to evaluate human papillomavirus genotype attribution
Objective To evaluate the necessity of HPV genotyping in cervical tissue samples by comparing the HPV detection results in formalin-fixed paraffin-embedded(FFPE)cervical tissues and cervical exfoliated cells.Methods A total of 101 patients who visited the Seventh Medical Center of the Chinese PLA General Hospital between 2021 and 2022 were randomly selected,including 21 cases of chronic cervicitis,20 cases of low-grade squamous intraepithelial lesions(LSIL),41 cases of high-grade squamous intraepithelial lesions(HSIL),and 19 cases of cervical squamous cell carcinoma(CSCC).Hybrid capture technology was used to compare the HPV detection results in cervical exfoliated cells and FFPE tissue specimens.Results The overall consistency rate of HPV DNA detection in cervical exfoliated cells and tissues was 78.2％.As cervical lesions progressed,the consistency of HPV results in cervical exfoliated cells and tissues significantly increased(P＜0.0001).Compared with cervical exfoliated cells,HPV detection in cervical tissues had higher specificity for cervical lesions(P＜0.0001).The most common HPV infection types in tissues were HPV16,52,58,18,and 33,which were more likely to cause cervical tissue lesions and potential effective HPV vaccine types.Conclusion HPV genotyping in cervical tissue samples can help evaluate the location and transmission status of viral infections and guide vaccine development.
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