Regulation of ovarian cancer SKOV3/A2780 cell proliferation and epithelial-mesenchymal transition by S100A2
Objective To investigate the role of S100A2 in regulating proliferation and epithelial-to-mesenchymal transition(EMT)processes in ovarian cancer(OC)cells SKOV3/A2780.Methods Gene expression datasets GSE14407 and GSE40595 were downloaded from the GEO database.Differential expression of S100A2 was identified using R software.In vitro experiments were conducted using epithelial OC cell lines SKOV3/A2780.Techniques such as siRNA,plasmid overexpression,RT-qPCR,and MTT assay were employed to study the expression and function of S100A2 in SKOV3/A2780 cells.Results Experimental result demonstrated higher expression levels of S100A2 in SKOV3/A2780 cells.Further functional assays revealed that knocking down S100A2 inhibited cell proliferation and reduced EMT.Conversely,overexpression of S100A2 enhanced cell proliferation and facilitated EMT.Mechanistically,modulating S100A2 expression influenced genes associated with the Wnt/β-catenin signaling pathway.Conclusion This study reveals the significant role of S100A2 in regulating biological characteristics of OC cell line SKOV3/A2780,offering a fresh perspective for better understanding OC pathogenesis and potential therapeutic targets.
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