Primary myelofibrosis of bone marrow pathological diagnosis of 58 cases
Objective To explore the pathological characteristics,immunophenotypes,diagnosis,and differential diagnosis of primary myelofibrosis(PMF).Methods A retrospective analysis was conducted on the bone marrow biopsy,Gomori,immunohistochemistry,JAK2 V617F,CALR exon 9,MPL exon 10,and BCR/ABL1 fusion gene detection results of 58 patients with PMF.Results The volume of hematopoietic tissue in 58 cases of bone marrow biopsy ranged from40％-90％,with 15.5％(9/58)being in the early PMF.79.3％(46/58)were in the overt PMF.5.2％(3/58)were in the overt PMF with osteosclerosis.Immunohistochemistry confirmed the distribution of granulosa,erythroid,and megakaryocyte lineages.Molecular detection of JAK2 V617F,CALR exon 9,and MPL exon 10 showed positive mutations in 70.7％(41/58).Conclusion Overt PMF is a common stage in bone marrow biopsy.Considering that it is difficult to distinguish early PMF from essential thrombocythemia and chronic myeloid leukemia,the diagnosis needs to be combined with clinical manifestations,histopathological characteristics,immunohistochemistry,and related gene testing for comprehensive diagnosis.
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