Bone marrow involvement of pleomorphic mantle cell lymphoma:clinicopathological analysis of nine cases
Objective To explore the clinicopathological features,immunophenotype,diagnosis,differential diagnosis of bone marrow involvement of pleomorphic mantle cell lymphoma(PMCL).Methods 9 cases of PMCL were studied by microscopic observation of bone marrow biopsy,immunohistochemical staining,flow cytometry and chromosome karyotypes.While the control groups included 13 cases of classic mantle cell lymphoma(CMCL)and 26 cases of diffuse large B-cell lymphoma(DLBCL).The related literatures were reviewed.Results The bone marrows were hypercelluar,the average proportion of neoplastic lymphocytes involving the bone marrow was 80％(70％-90％),and the pattern of bone marrow involvement of PMCL was diffuse.The tumor cells were large,nuclear chromatin was fine,some nucleoli were visible,and mitotic figures were less than 1/HPF.The tumor cells were positive for CD20,CD5,CD38,FMC7,CD79b,P53,CyclinD1,SOX11.Some cases expressed CD71,IgM,but in most cases lacked CD10,CD23,CD200,LEF1,c-myc,Bcl-6.The proliferation index Ki-67 was high.Conventional cytogenetic studies showed complex karyotype appeared in DLBCL cases more than PMCL cases.Conclusion The bone marrow involvement of PMCL is relatively rare.The correct diagnosis is based on clinicopathological features,immunophenotype and FISH-CCND1.
Pleomorphic Mantle Cell LymphomaImmunophenotypeBone marrow biopsyDiffuse Large B-cell Lymphoma
NETL
NSTL
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