Objective To explore the pathological features,immunophenotypes,diagnosis,and differential diagnosis of CML in bone marrow.Methods Retrospective analysis of bone marrow biopsy,gomon,immunohistochemistry,and BCR/ABL1 fusion gene detection results of 68 patients with CML,and review of relevant literature.Results There are 38 males and 30 females.68 cases of bone marrow biopsy showed a hematopoietic tissue volume between 60-90％,and 86.8％(59/68)showed significant proliferation of both granulocytes and megakaryocytes,belonging to the chronic phase of CML(double proliferation type of granulocytes and megakaryocytes).11.8％(8/68)showed significant proliferation of granulocytes,with no significant proliferation of megakaryocytes,belonging to the chronic phase of CML(granulocytes monoproliferative type).1.5％(1/68)showed significant proliferation of primitive granulocytes,belonging to the acute phase of CML.Immunohistochemistry confirmed the distribution of granulosa,erythroid,and megakaryocyte lineages,RT-PCR examination showed that the BCR/ABL1 fusion gene was 100％(68/68)positive for p210 fusion gene.Conclu-sion CML is commonly seen in the chronic phase of bone marrow biopsy pathology.Considering the difficulty in distin-guishing CML from PMF in the chronic phase,the diagnosis needs to be combined with clinical manifestations,histopatho-logical characteristics,immunohistochemistry,and BCR/ABL1 fusion gene testing for comprehensive diagnosis.
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