Correlation between HER2 expression and clinicopathological features in urothelial carcinoma
Objective To investigate the correlation of HER2 expression in urothelial carcinoma(UC)with its clinico-pathologic features,molecular types and prognosis.Methods A total of 115 UC patients admitted to the Eastern Theater Command General Hospital and Nanjing Drum Tower Hospital from May 2017 to February 2020 were retrospectively ana-lyzed.HER2 protein expression was detected by immunohistochemistry and HER2 gene amplification was detected by fluo-rescence in situ hybridization(FISH).Immunohistochemical markers GATA3,CK20,CK5/6 and CD44 were used for molecular typing of UC(luminal-like type,basal-like type and null type),and the relationship between HER2 expression and clinicopathological features and molecular typing was analyzed.Results The HER2 overexpression rate(IHC 3+)was 41.7％(48 cases),while the HER2 gene amplification rate was only 17.4％(20 cases).The coincidence rate of bladder urothelial carcinoma was 46.7％and that of upper urinary tract urothelial carcinoma was 25.0％.Of the 7 HER2-amplifica-tion positive cases,3 had an IHC score of 0 and 4 had an IHC score of 1+.The proportion of HER2 IHC positivity was highest in the micropapillary variant and lowest in the squamous variant.HER2 IHC-positive patients were more likely to develop lymph node metastasis.The expression rate of HER2 IHC was significantly higher than that of basal-like type and zero phenotype.The prognosis of IHC HER2-positive UC patients was poorer than that of IHC HER2-negative patients,but there was no statistically significant difference(P=0.099).There was no significant difference in prognosis between HER2 amplified and unamplified urothelial carcinoma patients(P=0.911).Conclusion The expression of HER2 in urothelial carcinoma is closely related to its molecular type,lymph node metastasis,and histological subtype.There is inconsistency between HER2 overexpression and gene amplification in urothelial carcinoma.
Urothelial carcinomaClinicopathologyHER2Fluorescence in situ hybridization
万方数据
维普
