Pathological diagnosis of bone marrow biopsy in primary thrombocytosis:analysis of 127 cases
Objective To explore the clinical and pathological characteristics,immunohistochemical phenotype,pathological diagnosis,and differential diagnosis of essential thrombocythemia(ET).Methods A retrospective analysis was conducted on 127 cases of ET newly-diagnosed at Beijing HST Medical Laboratory from 2021 to 2023,using bone marrow biopsy,immunohistochemistry,Gomori reticular fiber staining,molecular genetics,and other testing methods.Results The degree of bone marrow hyperplasia in 127 ET patients was as follows:active 66.1％(84/127),significantly active 32.3％(41/127),and extremely active 1.5％(2/127).CD61 confirmed a significant increase in megakaryocytes,with a large volume and deep or excessive nuclear lobulation;Gomori staining confirmed that MF-1 grade bone marrow reticular fiber tissue was more commonly detected;Molecular testing showed that the mutation rate of JAK2 gene was 73.2％(93/127),CALR gene mutation rate was 16.5％(21/127),MPL gene mutation rate was 0.4％(5/127),and gene mutation rate was 93.7％(119/127).Conclusion The degree of ET bone marrow hyperplasia is commonly normal or active,with an increase in megakaryocytes and MF-1 grade proliferation of reticular fibrous tissue.It is difficult to distinguish ET from primary myelofibrasis and polycythemia vera morphology.Diagnosis requires a comprehensive analysis of clinical manifestations,bone marrow biopsy,Gomori reticular fiber staining,immunohistochemistry,and related gene mutation.
万方数据
维普
