Association of core circadian clock gene polymorphism with ischemic stroke
Objective To explore the association between the single nucleotide polymorphism(SNP)of the core clock genes(ARNTL rs6486122 and PER1 rs2253820)of the circadian molecular clockwork and the incidence of ischemic stroke.Methods Candidate SNP genotypes were determined using the SNaPshot method.Genotypes were set as categorical vari-ables.Logistic regression analysis was performed to compare genotypes and genetic models between the disease group and the control group,adjusting for related risk factors including age,sex,dyslipidemia,and past medical history,and the adjusted odds ratios(OR)and 95%confidence intervals(CI)of different genotypes and genetic models were calculated.Results The multivariable logistic regression analysis showed that the risk of stroke increased significantly with the age of subjects(OR= 6.704,95%CI 5.188-8.644,P＜0.001);patients with hypertension had a 2.565-fold increased risk of ischemic stroke com-pared with individuals without hypertension(OR=2.565,95%CI 1.971-3.338,P＜0.001);patients with dyslipidemia were 1.700 times more likely to have ischemic stroke than individuals with normal blood lipid levels(OR=1.700,95%CI 1.230-2.348,P=0.001);and increased systolic and diastolic blood pressure levels also increased the risk of stroke.For the rs2253820 locus of the PER1 gene,using the wild-type homozygous TT genotype as the reference,the CT genotype,CC geno-type,and dominant genetic model significantly increased the risk of stroke(CT vs TT:OR=1.552,95%CI 1.194-2.018,P= 0.001;CC vs TT:OR=1.295,95%CI 1.035-1.621,P=0.024;and the dominant model:OR=1.563,95%CI 1.215-2.012,P=0.001,respectively).For the rs6486122 locus of the ARNTL gene,there was no significant association of any geno-type or the genetic model with the incidence of ischemic stroke after adjustment(P＞0.05).Conclusion Genetic variation at PER1 rs2253820 can increase the risk of ischemic stroke,where the C allele poses a higher risk of ischemic stroke.There is no significant association between ARNTL rs6486122 and the occurrence of ischemic stroke.
万方数据
维普
