Effect of hypoxic preconditioning on hypoxic injury of human umbilical vein endothelial cells
Objective To investigate the effect of hypoxic preconditioning(HPC)on human umbilical vein endothelial cells(HUVECs)at the cellular level(cell morphology,viability,and apoptosis rate),the molecular level[the expression of vascular endothelial growth factor(VEGF)and angiogenin-2(Ang-2)],and the genetic level(the expression of corresponding mRNAs),and to discuss the protective mechanism of HPC against hypoxic injury of HUVECs.Methods HUVECs were routinely cultured to establish a hypoxia model of HUVECs,and then HUVECs were divided into normal control group(CON group),hypoxia group(HYP group),and HPC group.Cell morphology was observed for each group under a microscope,and CCK-8 assay,Hoechst33258 fluorescent staining,Western blotting,and RT-PCR were used to measure cell viability,cell apoptosis rate,and the expression levels of VEGF,Ang-2,and corresponding mRNAs in cells.Results Compared with the CON group,the HYP group had significant damage of cells,a significant reduction in cell viability,and significant increases in cell apoptosis rate and the levels of VEGF,Ang-2,and corresponding mRNAs in cells,while the HPC group had basically normal cell morphology,significantly lower cell viability and apoptosis rate than the CON group,and significantly higher cell viability and apoptosis rate than the HYP group,as well as significantly higher expression levels of VEGF,Ang-2,and corresponding mRNAs than the CON group and the HYP group.Conclusion HPC exerts a protective effect against hypoxic injury of HUVECs by upregulating the expression of VEGF,Ang-2,and corresponding mRNAs.
万方数据
维普
