Serum proteomics in patients with cognitive impairment at high altitude
Objective To identify the differentially expressed proteins associated with cognitive impairment between the high-altitude population and the plain population,and to investigate the biological functions and signaling pathways of the differentially expressed proteins.Methods A total of 30 individuals living in the plain area(with an altitude of 400 m)and 30 individuals living in the high-altitude area(with an altitude of 3 960 m)were enrolled as plain group and high-altitude group,respectively,and general information was collected from all subjects.Montreal Cognitive Assessment(MoCA)was used to assess cognitive function.Blood samples were collected from each group,and the tims TOF Pro mass spectrometer was used to measure the serum levels of proteins after centrifugation.SPSS 25.0 was used for statistical analysis to investigate he association between proteomics and cognitive impairment.Results The results of MoCA assessment for both groups showed that the high-altitude group had a significantly lower MoCA score than the plain group,suggesting that there was significant cognitive impairment in the high-altitude group,with the main manifestation of impairment in visual space/executive ability,attention,delayed memory,and orientation.The proteomic analysis of serum samples from the subjects identified 169 differentially expressed proteins(84 upregulated proteins and 85 downregulated proteins),among which 39 proteins were associated with cognitive impairment.The enrichment analysis of the differentially expressed proteins showed that these differentially expressed proteins were involved in the regulation of multiple signaling pathways and metabolic pathways.Conclusion Significant cognitive impairment is observed in the high-altitude population,and there are differentially expressed proteins associated with cognitive function between the high-altitude population and the plain population.
万方数据
维普
