Sesn2 modulates LPS-induced macrophage M1 polarization and inflam-matory response by activating mTOR signaling pathway
AIM:To investigate the function of sestrin 2(Sesn2)in macrophages and its impact on macro-phage polarization and inflammatory response.METHODS:Knockdown of Sesn2 in RAW264.7 mouse macrophages was established using siRNA,and an in vitro inflammation model was created by treating RAW264.7 cells with lipopolysaccha-ride(LPS).The mRNA levels of interleukin-1β(IL-1β),IL-6,tumor necrosis factor-α(TNF-α),CD86 and inducible nitric oxide synthase(iNOS)in RAW264.7 cells were detected by RT-qPCR.The concentrations of IL-1β,IL-6 and TNF-α in the culture medium were measured by ELISA.The percentage of M1-type macrophages marked by CD86 was deter-mined by flow cytometry.The protein levels of mammalian target of rapamycin(mTOR),p70 ribosomal protein S6 kinase(p70S6K)and eukaryotic translation initiation factor 4E binding protein 1(4EBP1)were analyzed by Western blot.RE-SULTS:In the LPS-induced in vitro inflammation model,the expression levels of Sesn2,inflammatory factors(IL-1β,IL-6 and TNF-α),and M1 macrophage markers(CD86 and iNOS)in RAW264.7 cells were significantly increased,along with elevated protein expression of mTOR,p70S6K and 4EBP1.Silencing of Sesn2 with siRNA further enhanced these effects.However,if rapamycin was added before LPS in the experiment,these changes could be mitigated.CON-CLUSION:Sesn2 can regulate M1 macrophage polarization through mTOR/p70S6K/4EBP1 signaling pathway and affect the expression of pro-inflammatory factors.
国家科技期刊平台
NSTL
万方数据
维普
