Effect of Yunvjian on L cell injury induced by palmitic acid
ATM:To explore the impact of Yunvjian on palmitic acid(PA)-induced injury of intestinal L cells.METHODS:(1)Various concentrations(1.5,3 and 6 g/L)of Yunvjian solution and 1,1-diphenyl-2-picrylhydra-zyl(DPPH·)solution(100 mg/L)were incubated in darkness for 30 min.A full-wavelength microplate reader was em-ployed to measure the absorbance,thereby assessing the scavenging effect of Yunvjian on DPPH·.(2)Mouse intestinal endocrine cell line GLUTag was divided into control group,PA group,PA+Yunvjian(1.5 g/L)group,PA+Yunvjian(3 g/L)group,and PA+Yunvjian(6 g/L)group.The influence of Yunvjian on PA-induced apoptosis was ascertained through Hoechst 33342 nuclear staining and MTT assay.(3)The GLUTag cells were divided into control group,PA group,and PA+Yunvjian(6 g/L)group.The expression of glucagon-like peptide-1(GLP-1)was quantified by immunofluorescence staining.The level of reactive oxygen species(ROS)was determined using 2',7'-dichlorofluorescein diacetate(DCFH-DA)fluorescent probe,and superoxide dismutase 1(SOD1)protein level was analyzed by Western blot.RESULTS:(1)Yunvjian exhibited significant DPPH·scavenging activity in vitro(P<0.01).(2)Compared with PA group,Yunvjian markedly attenuated PA-induced decrease in GLUTag cell viability in a concentration-dependent manner,with the most pronounced effect observed at 6 g/L(P<0.01).(3)Compared with PA group,6 g/L Yunvjian significantly enhanced the expression of GLP-1(P<0.05)and SOD1(P<0.01),but reduced ROS level(P<0.01)in GLUTag cells.CONCLU-SION:Yunvjian potentially mitigates PA-induced injury in GLUTag cells by diminishing intracellular oxidative stress.
Yunvjianpalmitic acidintestinal L cellsoxidative stress