Expression of iNOS/CD163 in CD68+ cells from human chronic apical peri-odotitis tissues
AIM:To investigate the expression of inducible nitric oxide synthase(iNOS)and CD163 in peri-apical tissues at varying stages of human chronic apical periodotitis(CAP).METHODS:Forty-five voluntary samples were collected and categorized into three groups based on the degree of CAP:healthy control group(n=15),chronic apical abscess group(n=15),and periapical cyst group(n=15).Double immunofluorescence staining was employed to examine the expression of CD68+-iNOS+ and CD68+-CD163+ cells in periapical tissues.RESULTS:(1)The densities of CD68+-iNOS+ and CD68+-CD163+ cells showed significant differences among CAP groups(P<0.01).(2)The density of CD68+-iNOS+ cells in chronic apical abscess group was significantly higher than that in periapical cyst group(P<0.01).How-ever,there was no significant difference in the density of CD68+-CD163+ cells between chronic apical abscess group and periapical cyst group(P>0.05).(3)The ratio of CD68+-iNOS+/CD68+-CD163+ was significantly higher in chronic apical abscess group compared with periapical cyst group(P<0.01),while there was no significant difference in this ratio be-tween chronic apical abscess group and healthy control group(P>0.05).CONCLUSION:The expression ratio of CD68+-iNOS+/CD68+-CD163+ between chronic apical abscess and periapical cyst groups differed.Modulating the proportion of CD68+-iNOS+/CD68+-CD163+ may be involved in the development of CAP at various stages.
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