iNOS/CD163在人慢性根尖周炎组织中CD68+细胞上的表达
Expression of iNOS/CD163 in CD68+ cells from human chronic apical peri-odotitis tissues
王利宏 1刘园 2李娟 3黄世光3
作者信息
- 1. 广州中医药大学深圳医院(福田)口腔科,广东 深圳 518034
- 2. 广东省中医院,广东 广州 510120
- 3. 暨南大学口腔医学院,广东 广州 510632
- 折叠
摘要
目的:观察诱导型一氧化氮合酶(iNOS)和CD163在人不同类型的慢性根尖周炎组织中CD68+细胞的表达,分析在人不同类型的慢性根尖周炎组织中CD68+细胞的表达情况.方法:45名受试者按根尖周炎类型分为3组(健康对照组、慢性根尖脓肿组和根尖周囊肿组),每组15名.免疫荧光双染色法观察根尖周标本中CD68+-iNOS+和CD68+-CD163+细胞在各组标本中的表达情况,计算双阳性细胞的平均密度.结果:(1)3组标本中CD68+-iNOS+和CD68+-CD163+细胞密度差异显著(P<0.01);(2)慢性根尖脓肿组标本中CD68+-iNOS+细胞密度高于根尖周囊肿组(P<0.01),而两组间CD68+-CD163+细胞密度无显著差异(P>0.05);(3)慢性根尖周脓肿组中CD68+-iNOS+/CD68+-CD163+比值显著高于根尖囊肿组(P<0.01),而慢性根尖周脓肿组和对照组CD68+-iNOS+/CD68+-CD163+比值的无显著差异(P>0.05).结论:CD68+-iNOS+/CD68+-CD163+在慢性根尖脓肿组和根尖周囊肿组中表达的比值不同,调整其比值可能涉及慢性根尖周炎不同病程的发展.
Abstract
AIM:To investigate the expression of inducible nitric oxide synthase(iNOS)and CD163 in peri-apical tissues at varying stages of human chronic apical periodotitis(CAP).METHODS:Forty-five voluntary samples were collected and categorized into three groups based on the degree of CAP:healthy control group(n=15),chronic apical abscess group(n=15),and periapical cyst group(n=15).Double immunofluorescence staining was employed to examine the expression of CD68+-iNOS+ and CD68+-CD163+ cells in periapical tissues.RESULTS:(1)The densities of CD68+-iNOS+ and CD68+-CD163+ cells showed significant differences among CAP groups(P<0.01).(2)The density of CD68+-iNOS+ cells in chronic apical abscess group was significantly higher than that in periapical cyst group(P<0.01).How-ever,there was no significant difference in the density of CD68+-CD163+ cells between chronic apical abscess group and periapical cyst group(P>0.05).(3)The ratio of CD68+-iNOS+/CD68+-CD163+ was significantly higher in chronic apical abscess group compared with periapical cyst group(P<0.01),while there was no significant difference in this ratio be-tween chronic apical abscess group and healthy control group(P>0.05).CONCLUSION:The expression ratio of CD68+-iNOS+/CD68+-CD163+ between chronic apical abscess and periapical cyst groups differed.Modulating the proportion of CD68+-iNOS+/CD68+-CD163+ may be involved in the development of CAP at various stages.
关键词
慢性根尖周炎/CD68+细胞/诱导型一氧化氮合酶/CD163Key words
chronic apical periodotitis/CD68+ cells/inducible nitric oxide synthase/CD163引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据