干扰Gal-1通过TGF-β通路抑制人乳腺癌MDA-MB-231细胞的EMT和迁移
Interference with Gal-1 inhibits EMT and migration of human breast cancer MDA-MB-231 cells via TGF-β pathway
任世忠 1秦旭勇 1周国利 1赵薇 1曹淑俊 1苗振宇 1李成萍1
作者信息
- 1. 聊城大学生命科学学院,山东 聊城 252000
- 折叠
摘要
目的:探究干扰半乳糖凝集素1(Gal-1)通过转化生长因子β(TGF-β)途径对人乳腺癌MDA-MB-231细胞上皮-间充质转化(EMT)、迁移及增殖的影响和作用机制.方法:以shGal-1稳转细胞株和对照细胞株为材料,用Western blot检测TGF-β处理后shGal-1对MDA-MB-231细胞EMT进程的影响;通过细胞划痕实验和Transwell实验检测TGF-β处理后shGal-1对细胞迁移和侵袭的影响;用Western blot检测shGal-1对TGF-β通路蛋白的影响;用MTT实验和Western blot检测shGal-1对细胞增殖的影响.结果:shGal-1可抑制TGF-β介导的MDA-MB-231细胞EMT,并降低ERK、AKT和GSK3β的磷酸化水平,同时shGal-1可抑制MDA-MB-231细胞的迁移、侵袭和增殖.结论:shGal-1可抑制TGF-β介导的MDA-MB-231细胞EMT、迁移和增殖.
Abstract
AIM:To explore the effect and mechanism of the interfering Gal-1 on epithelial-mesenchymal transition(EMT),migration and proliferation in MDA-MB-231 cells via transforming growth factor-β(TGF-β)pathway.METHODS:The stable cell lines(shGal-1)which Gal-1 expression were inhibited completely and their control cell lines were used as experimental cells.Western blot assay was used to detect the effects of shGal-1 on EMT process of MDA-MB-231 cells after TGF-β treatment;The effect of shGal-1 on cell migration and invasion after TGF-β treatment was verified by cell scratch and transwell test;The effect of shGal-1 on the TGF-β pathway related proteins were detected by western blot;Finally,the effect of shGal-1 on cell proliferation was detected by MTT and western blot.RESULTS:shGal-1 inhib-ited TGF-β-mediated EMT in MDA-MB-231 cells and regulated phosphorylation of pathway signaling molecules(ERK,AKT and GSK3β);shGal-1 could inhibit the proliferation of MDA-MB-231 cells.CONCLUSION:shGal-1 can inhibit the TGF-β-mediated EMT,migration and proliferation of MDA-MB-231 cells.
关键词
半乳糖凝集素1/上皮-间充质转化/转化生长因子β/MDA-MB-231细胞/细胞迁移/细胞增殖Key words
Galectin-1/epithelial-mesenchymal transition/transforming growth factor-β/MDA-MB-231 cells/cell migration/cell proliferation引用本文复制引用
基金项目
山东省自然科学基金(ZR2020QC064)
聊城大学科技处博士科研启动资金项目(318051604)
出版年
2024
国家科技期刊平台
NSTL
万方数据