维生素D受体在肺腺癌高表达及其对肺腺癌细胞活力和转移能力影响的研究
High expression of vitamin D receptor in lung adenocarcinoma and its in-fluence on viability and metastasis ability of lung adenocarcinoma cells
邹淑梅 1包振明 1叶嘉 1余宗阳 2宋颖芳3
作者信息
- 1. 联勤保障部队第900医院呼吸内科,福建 福州 350025
- 2. 福建医科大学福总临床医学院,福建 福州 350025;联勤保障部队第900医院呼吸内科,福建 福州 350025
- 3. 厦门大学附属东方医院,福建 厦门 361000;福建医科大学福总临床医学院,福建 福州 350025;联勤保障部队第900医院呼吸内科,福建 福州 350025
- 折叠
摘要
目的:探究维生素D受体(vitamin D receptor,VDR)在肺腺癌中的表达及其对肺腺癌细胞生物学功能的影响.方法:本研究通过6个肺腺癌数据集(共792例肺腺癌组织和230例相邻非肿瘤组织)分析了VDR的mRNA在肺腺癌中的表达水平及其与预后的关系,免疫组化检测30例肺腺癌患者肿瘤组织和癌旁组织中VDR蛋白表达.以肺腺癌细胞A549和H1650为研究对象,分别转染阴性对照和两条VDR短发夹RNA(shRNA).CCK-8实验比较各实验分组的细胞活力,Transwell实验和划痕实验比较各实验分组细胞的侵袭及迁移能力.基因集富分析肺腺癌VDR高表达的组织富集的相关信号通路.结果:VDR的mRNA在肺腺癌组织中的表达显著增加(P<0.01).免疫组化实验进一步证实了VDR在肺腺癌中显著高表达(P<0.01).生存分析结果显示VDR的表达对肺腺癌患者的整体生存率没有显著影响(P>0.05).敲减VDR可以显著抑制肺腺癌的细胞活力、侵袭及迁移能力(P<0.05).基因集富集分析结果显示VDR高表达的肺腺癌组织显著富集在上皮-间充质转化等信号通路(P<0.05).结论:VDR在肺腺癌中高表达,敲减VDR可以抑制肺腺癌细胞活力、侵袭及迁移能力.
Abstract
AIM:To explore the expression level of vitamin D receptor(VDR)in lung adenocarcinoma and its impact on the biological function of lung adenocarcinoma cells.METHODS:The mRNA expression level of VDR in lung adenocarcinoma and its relationship with prognosis were analyzed through 6 lung adenocarcinoma datasets(compris-ing a total of 792 lung adenocarcinoma tissues and 230 adjacent non-tumor tissues).Immunohistochemistry was used to de-tect VDR protein expression in 30 lung cancer patients.Lung adenocarcinoma cell lines A549 and H1650 were studied,with transfection of negative control(NC)or two VDR short hairpin RNAs(VDR-shRNAs).CCK-8 assay compared the cell viability of cells in each experimental group.Transwell and wound-healing assays compared the invasion and migra-tion capabilities.Gene set enrichment analysis identified pathways enriched in lung adenocarcinoma tissues with high VDR expression.RESULTS:The mRNA expression level of VDR was significantly increased in lung adenocarcinoma tissues(P<0.01).Immunohistochemistry further confirmed the high expression of VDR in lung adenocarcinoma(P<0.01).The survival analysis showed that the expression of VDR had no significant effect on the overall survival of lung ad-enocarcinoma patients(P>0.05).Knockdown of VDR significantly inhibited the cell viability,invasion,and migration ca-pacity of lung adenocarcinoma cells(P<0.05).Gene set enrichment analysis showed that lung adenocarcinoma tissues with high VDR expression were enriched in signaling pathways such as epithelial-mesenchymal transition(P<0.05).CONCLUSION:VDR is highly expressed in lung adenocarcinoma,and VDR knockdown can inhibit the cell viability,in-vasion,and migration capacity of lung adenocarcinoma cells.
关键词
维生素D受体/肺腺癌/细胞活力/细胞侵袭/细胞迁移Key words
vitamin D receptor/lung adenocarcinoma/cell viability/cell invasion/cell migration引用本文复制引用
基金项目
福建省自然科学基金面上项目(2020J011137)
联勤保障部队第九〇〇医院临床医学研究专项(2020L13)
出版年
2024
国家科技期刊平台
NSTL
万方数据