首页|葛根-丹参对糖尿病相关动脉粥样硬化小鼠脂肪因子稳态的影响

葛根-丹参对糖尿病相关动脉粥样硬化小鼠脂肪因子稳态的影响

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:探究葛根-丹参通过调节脂肪因子(adipokine)稳态干预糖尿病(diabetes mellitus，DM)相关动脉粥样硬化(atherosclerosis，AS)小鼠的机制。方法:对雄性载脂蛋白E基因敲除(apolipoprotein E gene knock-out，ApoE-/-)小鼠(C57BL/6J背景)以高脂饲料喂养联合链脲佐菌素腹腔注射构建DM相关AS模型，造模成功后随机分为模型组、低剂量葛根-丹参组、高剂量葛根-丹参组和阳性对照药物二甲双胍组，另以雄性C57BL/6J小鼠为空白对照组，每组6只。油红O染色法观察主动脉病理形态学变化;HE染色观察腹腔脏器脂肪组织形态;全自动生化分析仪检测血清中总胆固醇(total cholesterol，TC)、甘油三酯(triglyceride，TG)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol，HDL-C)及低密度脂蛋白胆固醇(low-density lipoprotein cholesterol，LDL-C)水平;ELISA法检测血清中胰岛素水平;RT-qPCR及Western blot法检测脂肪组织中脂肪因子[脂联素(adiponectin)、爱帕琳肽(ape-lin)、趋化素(chemerin)和抵抗素(resistin)]的蛋白表达情况。结果:与空白对照组相比，模型组小鼠脂肪组织中adiponectin和apelin蛋白表达水平显著降低(P＜0。01)，chemerin和resistin蛋白表达水平显著升高(P＜0。01);与模型组相比，高剂量葛根-丹参组小鼠脂肪组织中adiponectin和apelin蛋白表达水平显著升高(P＜0。01)，chemerin和re-sistin蛋白表达水平显著下降(P＜0。01)，且胰岛素分泌显著增加(P＜0。01)，LDL-C表达水平显著降低(P＜0。01)。油红O染色结果显示，高剂量葛根-丹参组小鼠主动脉AS斑块较模型组减少且面积较小。结论:葛根-丹参药对可能促进DM相关AS小鼠脂肪组织中adiponectin和apelin表达，抑制chemerin和resistin表达，调节脂肪因子稳态，同时参与调节糖脂代谢，从而发挥抗AS作用。
Effect of Puerariae lobatae Radix and Salviae miltiorrhizae Radix et Rhi-zoma on adipokine homeostasis in mice with diabetes-related atheroscle-rosis
AIM:This study aims to investigate the mechanisms by which Gegen(Puerariae lobatae Radix)and Danshen(Salviae miltiorrhizae Radix et Rhizoma)intervene in diabetes mellitus(DM)-related atherosclerosis(AS)through the regulation of adipokine homeostasis.METHODS:A mouse model of DM-related AS was established in male apolipoprotein E gene knockout(ApoE-/-)mice by high-fat diet feeding and intraperitoneal injection of streptozotocin.The mice were randomly divided into model group,low-dose Gegen-Danshen group,high-dose Gegen-Danshen group and posi-tive control drug metformin group,while male C57BL/J mice served as blank control group,with 6 mice in each group.Se-rum lipid levels of the mice were assessed.Oil red O staining was employed to observe pathological changes in the abdomi-nal aorta,while HE staining was used to examine the morphological changes of adipose tissues surrounding abdominal or-gans.The protein expression levels of adipokines,including adiponectin,apelin,chemerin and resistin,in adipose tis-sues were measured using RT-qPCR and Western blot.RESULTS:The administration of Gegen and Danshen resulted in a significant increase in the protein expression levels of adiponectin and apelin in adipose tissues(P＜0.01).Conversely,the levels of chemerin and resistin were significantly decreased(P＜0.01),and there was a notable reduction in low-densi-ty lipoprotein cholesterol(P＜0.01).Results of oil red O staining revealed that the aortic AS plaques of the mice in model group were numerous,large and scattered,whereas those in high-dose Gegen-Danshen group were fewer and smaller.CONCLUSION:Gegen and Danshen play a protective role in preventing DM-related AS in mice by enhancing the expres-sion of adiponectin and apelin but inhibiting the expression of chemerin and resistin.

Puerariae lobatae RadixSalviae miltiorrhizae Radix et Rhizomadiabetes mellitusatheroscle-rosisadipokine

乔羽、王晓磊、吴长江、孙金旭、李文慧、郭翔宇

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北京中医药大学东方医院,北京 100078

葛根 丹参 糖尿病 动脉粥样硬化 脂肪因子

2024

10.3969/j.issn.1000-4718.2024.12.006

中国病理生理杂志
中国病理生理学会

中国病理生理杂志

CSTPCD北大核心
影响因子：1.065
ISSN：1000-4718
年,卷(期)：2024.40(12)