ISSR Analysis and Genetic Diversity Research of Different Alfalfa Materials
The genetic diversity of twelve alfalfa materials were investigated with ISSR marker.A total of 346 bands were amplified with 13 ISSR primers,there were 332 polymorphic bands.The average percentage of polymorphic bands were 95.95%.331 were polymorphic (PPB =95.66%) in species level.The results of popgen32 analysis indicated that Nei's gene diversity (H*) was 0.1599,Shannon information index (I*) was 0.2098.Gene difference index (Gst) among materials was 0.3114,which means the percentage of gene difference among materials was only 31.14%.The result showed that the genetic variation within materials was higher than among materials.The cluster analysis showed that Medicago sativa was closer to Medicago varia,while it is the widest to Medicago falcata.