In order to study the function of calcium-dependent protein kinase (CDPK) in L.chinensis adapted to habitats,seedlings were treated respectively with simulation of drought (polyethylene glycol,PEG) and saline-alkali (NaCl-Na2 CO3).This test sequenced and analyzed the cDNA of CDPK cloned from L.chinensis (LcCDPK).The nucleic acid sequence length was 1704 bp,with an open reading frame (ORF) of 1647 bp,encoding 548 amino acids.The encoding amino acid sequence had the highest homology with CDPK1 of wheat from GenBank(96％).The results of bioinformatics soft wares showed:the amino acid sequence has a S-TKc structural domain with the catalytic activity of serine/threonine protein kinase,and there were four conservative EF-hands structural domain with binding Ca2+.It was predicted that relative molecular mass of mature peptide was 61.349 KDa and theoretical isoelectric point was 5.34,meanwhile,in secondary structure,alpha-helix accounted for 37.59％,beta-fold 9.85％,random coil 52.55％.The results of real-time PCR showed:CDPK gene expression in roots and leaves all were down-regulating under low concentration PEG or saline-alkali treatment conditions while its expression was up-regulating under high concentration conditions.CDPK gene expression and regulation were impacted by drought and saline-alkali stress.CDPK gene expression with saline-alkali treatment all was higher than that of drought treatment in roots and leaves.Therefore,it is speculated that LcCDPK widely participate in defending stress in L.chinensis,and especially play an important role in the process of saline-alkali stress.
NETL
NSTL
万方数据
维普
