Cloning of PpSWEET1b Gene from Poa pratensis and Its Functional Verification in Arabidopsis thaliana
SWEETs sugar transporters are one of the sugar transporters that promoted sugar flow through cell membranes in both eukaryotic and prokaryotic,and are involved in regulating plant growth,development,and stress responses.The PpSWEET1b gene was cloned from the leaf cDNA of Poa praten-sis cv.Blue moon as a template,and transformed into Arabidopsis thaliana by inflorescence infection method to obtain overexpressed PpSWEET1b strains.The effects of drought and low temperature stress on the growth,physiology,and gene expression of transgenic and wild-type Arabidopsis thaliana were studied.The results showed that under drought and low temperature stress,the growth phenotype of PpSWEET1b overexpressing plants was better than that of the wild type,and the MDA content in leaves was significantly lower than that of the wild type.However,the glucose content was significantly increased compared to the wild type,and the drought and cold resistance were significantly enhanced;PpSWEET1b overexpressing plants may collaborate with SWEET2 and SWEET3 to participate in the response to low temperature.In addition,the promoter sequence of the PpSWEET1b gene was cloned using leaf DNA as a template,and cis acting elements were predicted to contain multiple elements related to stress,hormone response,and light response.These results indicated that PpSWEET1b gene of Poa pratensis plays an important role in drought and cold tolerance,and can provide new excellent gene resources for biological breeding of turf grass to resist abiotic stress.
万方数据
维普
