中国草地学报2024,Vol.46Issue(1) :56-69.DOI:10.16742/j.zgcdxb.20230109

9个裸燕麦品种苗期耐低氮性评价

Evaluation of Nitrogen Tolerance in Nine Naked Oat Varieties:A Seeding Stage Study

张心怡 赵新玥 田莉华 王沛 周青平 雷映霞
中国草地学报2024,Vol.46Issue(1) :56-69.DOI:10.16742/j.zgcdxb.20230109

9个裸燕麦品种苗期耐低氮性评价

Evaluation of Nitrogen Tolerance in Nine Naked Oat Varieties:A Seeding Stage Study

张心怡 1赵新玥 1田莉华 1王沛 1周青平 1雷映霞1
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作者信息

  • 1. 西南民族大学四川若尔盖高寒湿地生态系统国家野外科学观测研究站,四川 成都 610041
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摘要

为探究裸燕麦在低氮条件下的生长和生理响应情况,评价不同品种的耐低氮能力,本研究以9个裸燕麦品种为试验材料,设置全氮(4 mmol/L,CK)和1/10 N(0.1 mmol/L,低氮)两个氮浓度,在苗期处理21 d后对裸燕麦的形态指标和生理指标进行测定.结果表明:在低氮条件下,相比对照组:(1)裸燕麦的根长、生物量均有不同程度下降,其中WDY5的地上部干重降幅最大,同时裸燕麦的株高除ZY8呈上升趋势外其他均呈下降趋势;(2)裸燕麦的硝态氮、硝酸还原酶、谷氨酰胺合成酶、谷氨酸脱氢酶活性、渗透调节物质的含量均呈下降趋势,谷氨酸合成酶活性、超氧阴离子、过氧化氢、丙二醛含量指标呈上升趋势,其中QY3的丙二醛含量升幅最小;(3)主成分分析将21个测定指标转换为5个主成分,累计方差贡献率为91.76%;并进行聚类分析,结果将9个裸燕麦品种划分为3个类群:耐低氮品种3个、中等耐低氮品种5个、低氮敏感型品种1个;计算耐低氮Z值对其耐低氮性进行综合评价,筛选出耐低氮品种为QY3,氮敏感品种为WDY5.

Abstract

This study aimed to investigate the growth,physiological responses,and nitrogen tolerance of nine naked oat(Avena nuda L.)varieties under low nitrogen(0.4 mmol/L,LN)and control(4 mmol/L,CK)conditions for 21 days.Morphological and physiological indices were measured and analyzed.Under low nitrogen conditions,compared to the control group,the results showed as follows:(1)Root length and bio-mass decreased,with WDY5 exhibiting the largest decline in shoot dry weight.Plant height decreased in all varieties except ZY8.(2)Nitrate assimilation-related enzymes(nitrate reductase glutamine synthase,gluta-mate dehydrogenase),osmoregulatory substances,and nitrogen content decreased,while stress markers(glutamate synthetase activity,superoxide anion,hydrogen peroxide,malondialdehyde)increased,with QY3 showing the least malondialdehyde accumulation.(3)Principal component analysis revealed five princi-pal components explaining 91.76%of the variance contribution.Cluster analysis categorized the varieties into three groups:three low-nitrogen tolerant,five medium-low-nitrogen tolerant,and one low-nitrogen-sensitive(WDY5).The Z-value analysis confirmed QY3 as the most tolerant and WDY5 as the most sensi-tive variety to low nitrogen stress.

关键词

裸燕麦/低氮条件/生理响应/综合评价

Key words

Naked oat/Low nitrogen conditions/Physiological response/Comprehensive evaluation

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基金项目

四川省自然科学基金青年基金(2023NSFSC1134)

中央高校基本科研业务费(CX2023011)

出版年

2024
中国草地学报
中国农业科学院草原研究所 中国草地学会

中国草地学报

CSTPCD北大核心
影响因子:1.347
ISSN:1673-5021
参考文献量22
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