摘要
以老芒麦(Elymus sibiricus)为研究材料,探讨了不同浓度外源SA(0.25、0.5、1.0、1.5和 2.0 mmol/L)对NaCl胁迫下种子萌发及幼苗生长的影响.结果表明:在150 mmol/L NaCl胁迫下,外源添加0.25、0.5、1.0 mmol/L的SA均能减缓盐胁迫对老芒麦造成的抑制作用.综合评价分析表明,外源添加0.25 mmol/L的SA效果最为显著(P<0.05),此时老芒麦的种子发芽势、根冠比、幼苗可溶性蛋白含量和POD活性分别提高了32.58%、21.15%、69.48%和81.09%,而丙二醛含量降低了73.40%.本研究表明在盐胁迫环境下,外源SA能通过提高老芒麦的抗氧化酶活性和消除由ROS引起的氧化损伤等方式维持其正常生长.本研究可为老芒麦在盐渍化土壤地区的推广、应用及生态治理提供一定的技术参考.
Abstract
This study aimed to investigate the effects of exogenous salicylic acid(SA)on seed germina-tion and seedling growth of Elymus sibiricus under NaCl stress.The study comprised five groups,includ-ing the control group(distilled water,CK)the exogenous salicylic acid(SA)treatments at concentrations of 0.25 mmol/L,0.5 mmol/L,1.0 mmol/L,1.5 mmol/L,and 2.0 mmol/L.The results showed that exogenous SA treatments at concentrations of 0.25,0.5,and 1.0 mmol/L alleviated the inhibitory effect of salt stress(150 mmol/L)on E.sibiricus.Comprehensive evaluation results revealed that the addition of exogenous SA at 0.25 mmol/L had the optimum effect(P<0.05).Under this concentration,seed germi-nation energy,shoot-to-root ratio,soluble protein content,and peroxidase(POD)activity of E.sibiricus increased by 32.58%,21.15%,69.48%and 81.09%,respectively.Additionally,the malondialdehyde(MDA)content decreased by 73.40%.In conclusion,exogenous SA can maintain the normal growth of E.sibiricus under NaCl stress by increasing the activity of antioxidant enzymes and eliminating the oxida-tive damage caused by reactive oxygen species(ROS).This study provides technical reference for the pro-motion and ecological application of E.sibiricus in salinized soil areas.
基金项目
四川省重点研发项目(2023YFN0087)
四川省肉牛产业体系创新团队项目(SCCXTD-20-13)
国家自然科学基金项目(3177131276)
维普
万方数据