The aim of the study was to investigate the effects of exogenous melatonin on the growth char-acteristics and antioxidant of bermudagrass(Cynodon dactylon L.)under lead stress.C.dactylon cv.Xin-nong No.1 and C.dactylon×C.trasvalensis cv.Tifgreen were used as materials.After 20 days growth of bermudagrass stems,they were treated with 0,5,20 and 50 μmol/L melatonin,and then treated with lead acetate solution at the concentrations of 0,1000 and 2000 mg/kg.The results showed that before lead stress,the relative water content,soluble protein,and antioxidant enzymes contents of Cynodon dactylon cv.Xin-nong No.1 were significantly higher than those of C.dactylon×C.trasvalensis cv.Tifgreen(P<0.05).Lead stresses inhibits the growth of bermudagrass and increased cellular oxidative stress response.Under lead stress,compared with the control,the bermudagrass pretreated with 20 μmol/L or 50 μmol/L concentra-tions of melatonin showed higher antioxidant enzymes activity and non-enzymatic antioxidants substance content,and increased chlorophyll and relative water content.It can be seen that melatonin enhanced the antioxidant enzyme activity of bermudagrass and enhanced the antioxidant defense system to eliminate free radicals produced under lead stress,which can provide theoretical support and practical basis for the applica-tion of exogenous melatonin to improve the remediation of lead contaminated soil by bermudagrass.
关键词
重金属/叶绿素/渗透调节/抗氧化酶/非酶抗氧化物质
Key words
Heavy metal/Chlorophyll/Osmoregulation induced/Antioxidant enzymes/Non-enzymatic antioxidants
维普
万方数据