Isolation and Identification of Endophytic Bacterium B7 from Caucasian Clover Seeds and Its Growth Promotion Characteristics
The endophytic bacterium B7 was isolated and screened from the seeds of Caucasian clover by traditional isolation and culture methods.The molecular identification was performed by physiological and biochemical characteristics and 16S rDNA,and the results showed that B7 belonged to the genus Pano-bacterium(Pantoea sp.).The strain B7 could grow normally under 15%PEG6000 drought stress,show-ing strong drought tolerance.It could grow in pH range of 5 to 11,with good acid and alkali tolerance.It had the ability to produce IAA,secrete iron carrier,fix nitrogen and dissolve phosphorus,among which the IAA production ability was as high as 0.28 g/L,the dissolution indexes of organic phosphorus,inorganic phosphorus and iron carrier were 3.15,2.91 and 2.63,and the rates were 2.15,1.91 and 1.63,respec-tively.The strain B7 had good growth-promoting effect,which significantly improved the plant height,aboveground fresh weight,underground fresh and dry weight,leaf length,leaf width,number of leaves,leaf area,root length,root projected area,root surface area as well as number of root tips of Caucasian clover and promoted the growth and development of Caucasian clover.
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