Effects of Sucrose Addition on Fungal Community Diversity in High-moisture Alfalfa Silage
This study aimed to investigate the diversity of the fungal community in high-moisture alfalfa silage,both in the control group(without sucrose supplementation)and in the sucrose group(supple-mented with sucrose),using metagenomic sequencing.The results showed that,in comparison to the con-trol group,the addition of sucrose significantly increased the lactic acid content(P<0.05),while signifi-cantly reducing the concentrations of acetic acid,butyric acid,and ammonia nitrogen,as well as the popula-tion of aerobic bacteria(P<0.05).Ascomycetes were identified as the most abundant fungal phyla in both alfalfa and its silage,followed by Basidiomycetes.Genera such as Moriella and Streptospora dominated the fungi community in alfalfa,whereas Beauveria was the most abundant genus in both groups of silage.Aspergillus and Penicillium exhibited relatively low abundances.In comparison to the control group,the relative abundances of Penicillium,Aspergillus,and Alternaria in the sucrose group were reduced by 24.59%,1.49%,and 0.22%,respectively.The dominant fungi species in alfalfa were Tenebrio Moesei and Streptospora interseptum,while Beauveria bassiana dominated the two groups of silage.Conversely,the relative abundance of Aspergillus flavus was low.Furthermore,the relative abundances of Bassiana bassiana and Aspergillus flavus showed a negative correlation with lactic acid content and a positive correla-tion with the contents of acetic acid,butyric acid,and ammonia nitrogen.In conclusion,the sucrose addi-tion group exhibited superior fermentation quality.Melonella australis and Beauveria bassiana were identi-fied as the dominant fungal populations in high-moisture alfalfa and its silage,respectively.The addition of sucrose can inhibit the propagation of Beauveria bassiana to a certain extent.
万方数据
维普
