Analysis of the Transcription Pattern and Resistance Function of LpPIL15-like Gene in Perennial Ryegrass
Phytochrome interacting factors(PIFs)belong to the bHLH family and play crucial roles in plant light signal transduction,photomorphogenesis and stress resistance.Based on the published transcrip-tome data of perennial ryegrass,we cloned a stress-responsive differentially expressed PIF gene named LpPIL15-like,and preliminarily analyzed its gene characteristics,transcription patterns and stress resis-tance function.LpPIL15-like gene has 5 exons,a total coding length of 1671 bp,and encoding 556 amino acids,which is the closest genetic relationship with the LrPIL15-like gene of Lolium rigidum.LpPIL15-like protein has a typical bHLH domains and APB and APA functional domains,and is located in the nucleus.The promoter of LpPIL15-like contains hormones,stress and light response cis-regulatory ele-ments such as ABRE,CGTCA motif and G-box.Quantitative analysis showed that the expression of LpPIL15-like was the highest in extended leaves,and the expression was lower in stem,root and leaf sheaths.Furthermore,the expression of LpPIL15-like gene was regulated by circadian rhythm,and the expression of LpPIL15-like gene was significantly higher under light than under dark conditions.PEG,NaCl,CdCl2,and high temperature stress and 6-BA,ABA and other hormones treatment at 0.5,2,6,12,and 24 h significantly enhanced the expression of LpPIL15-like in leaves,while stress and hormone treatments at 0.5,2,6,and 12 h significantly inhibited the expression of LpPIL15-like gene in roots.Het-erologous overexpression of LpPIL15-like significantly improved the salt,high temperature and drought tol-erance of yeast.In conclusion,the transcription of LpPIL15-like is regulated by circadian rhythm,abiotic stress and plant hormone,and can be used as a candidate gene for genetic improvement of stress tolerance in ryegrass.
NETL
NSTL
万方数据
维普
