Metabolomic Analysis of Medicago sativa at Germination and Seedling Stage under Salt Stress
Alfalfa(Medicago sativa)is an excellent plant material for saline-alkali land bio-improvement.The germination and seedling stages are the key stages for its planting in saline-alkali land.However,there are few reports on the metabonomic analysis of alfalfa under salt stress at the germination and seedling stages.In order to deeply and comprehensively understand the metabolic regulation mecha-nism of salt tolerance in alfalfa,0(control),50,100,200 and 400 mmol/L NaCl stress were set to deter-mine the emergence rate,survival rate,plant height and root length of alfalfa,the root metabolism of alfalfa at germination and seedling stages under control and 100 mmol/L NaCl stress was analyzed by high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS).The results showed that alfalfa had a certain salt tolerance.When the salt concentration was≤100 mmol/L,there was no signifi-cant difference in seedling emergence rate and survival rate of alfalfa compared to the control treatment.205 differential metabolites were identified at the germination stage of alfalfa under 100 mmol/L salt stress.Among them,48 differential metabolites were enriched in 55 KEGG metabolic pathways.The differential metabolites were mainly enriched in pathways such as ABC transporter,phosphatidylinositol signaling sys-tem,and amino acid metabolism.The differential metabolites inositol and L-tyrosine in these pathways were significantly upregulated.A total of 108 differential metabolites were identified at the seedling stage of alfalfa,of which 24 were enriched in 37 KEGG metabolic pathways.Differential metabolites were mainly enriched in plant hormone signal transduction and biosynthesis of other secondary metabolites.The differential metabolites in these pathways,indole-3-acetic acid,L-tyrosine,and piperidine,were signifi-cantly upregulated.
Salt stressGermination stageSeedling stageMetabolomicsMedicago sativa
NETL
NSTL
万方数据
