Screening and Validation of qRT-PCR Reference Genes in Chenopodium album under the Stress of Aureobasidium pullulans PA-2
In order to screen the stable expression of reference genes in Chenopodium album under the stress of Aureobasidium pullulans PA-2,based on the analysis of transcriptome data(NCBI access number:SRA:SRR12674257),the expression levels of 12 reference genes in Chenopodium album under different time stress of PA-2 were analyzed,including ACT-1,TUB-1,GAPDH,eEF1α,eIF3,UBQ,PTB,COX10,UPL6,TIP41L,RAN-B1 and IDH-5.The expression stability of candidate reference genes was analyzed by ΔCt,GeNorm,NormFinder,BestKeeper and RefFinder.The results revealed that the reference gene COX10 was the most stable reference gene in Chenopodium album under different time stress of PA-2,and the optimal combination was COX10+eEF1α.The reliability of the reference genes was verified by ana-lyzing the relative expression levels of acetyl-CoA carboxylase gene(ACC)in C.album.The findings of the present study provide reference genes and theoretical basis for the expression analysis of stress response gene in C.album in the future.
NETL
NSTL
万方数据
维普
