Effects of Exogenous Salicylic Acid on Sainfoin Growth and Physiological Characteristics under Sodium Chloride Stress
This study investigated the impact of exogenous salicylic acid(SA)on the growth and physi-ological traits of sainfoin(Onobrychis viciaefolia)seedlings under salt stress,aiming to inform breeding strategies for enhanced salt resistance.A sand culture system with 0.8%sodium chloride(NaCl)treat-ment was employed.Salt-sensitive(1 and'Mengnong')and salt-tolerant(9860 and 9970)were foliar-sprayed with different SA concentrations(0.5,1.0,1.5 mmol/L)to determine the optimal mitigation dose.The results showed that SA significantly increased plant height,above-ground biomass,below-ground biomass,root activity,and leave/root K+ content of sainfoin seedlings under salt stress.Con-versely,it reduced soluble sugars,soluble proteins,proline,malondialdehyde,Na+ content of leave/root,and catalase,peroxidase,and superoxide dismutase activities.These findings suggest SA mitigates salt stress by reducing membrane lipid peroxidation and maintaining membrane system integrity.Among the SA concentration tested,1.0 mmol/L demonstrated the most significant alleviation of salt damage.The results provide a scientific basis for the rational application of SA to improve salt tolerance in sainfoin culti-vation.