Cloning,Expression and Transcriptional Autoactivation Analysis of MtMYB16 Gene in Medicago truncatula
Myeloblastosis(MYB)transcription factors in plants are abundant and versatile,and play an important role in plant growth and development,stress response,and other aspects.In order to study the function of MYB transcription factor in Medicago truncatula,the MtMYB16 gene was cloned from Medi-cago truncatula by PCR technology.The gene has an open reading frame of 1074 bp and encodes a total of 357 amino acids.The evolutionary tree analysis results showed that MtMYB16 protein had the highest homology with MYB protein in Melilotus albus.Subcellular localization results exhibited that MtMYB16 protein was localized in the nucleus.Yeast autoactivation experiments indicated that MtMYB16 protein had autoactivation activity,and its autoactivation domain was located at C terminus.Expression analysis results showed that MtMYB16 was expressed in roots,stems,leaves,flowers and fruits of Medicago trun-catula,and the highest expression level was in roots.Both IAA and MeJA could reduce the expression level of MtMYB16.Under salt and drought stress,the expression level of MtMYB16 increased rapidly at 1.0 h,indicating that MtMYB16 may have a responsive function to salt and drought stress.
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