美洲狼尾草CCD基因家族全基因组鉴定及分析
Genome-wide Identification and Analysis of the Carotenoid Cleavage Dioxygenase(CCD)Gene Family in Pennisetum Glaucum
王净洁 1阿布都沙拉木 1祝诗词 1叶歆雅 1范欲航 1黄琳凯1
作者信息
- 1. 四川农业大学草业科技学院,四川 成都 610000
- 折叠
摘要
鉴定美洲狼尾草(Pennisetum glaucum)中的类胡萝卜素裂解双加氧酶(Carotenoid cleavage dioxygenases,CCD)基因家族成员,为美洲狼尾草中PmCCD基因进一步的功能研究与遗传改良提供一定的理论依据.基于美洲狼尾草已公开的全基因组数据,成功鉴定了其中的CCD成员,并预测了该家族成员的系统进化关系,此外,分析了这些成员的蛋白理化性质、功能结构以及保守基序,并深入研究了它们的启动子顺式作用元件等,最后结合转录组数据分析了PmCCD基因在组织中、高温胁迫和干旱胁迫下的表达模式.共鉴定出13个PmCCD基因,分布于5条染色体上,系统进化分类为2个大类别,共计6个亚家族,所鉴定出的成员均具备典型的RPE65或PLN型蛋白结构域,其启动子区域有与逆境胁迫、激素响应以及生长发育等生物过程密切相关的顺式作用元件.转录组数据分析表明,PMccd-1、PMcccd-6、PMccd-7、PMccd-12基因在美洲狼尾草的穗组织中具有显著的高表达,在干旱与高温胁迫下,PmCCD基因产生了显著的表达变化,且受胁迫影响根系中的表达差异高于叶片.综上结果表明在干旱诱导时,Pmccd-12、Pmccd-6基因分别在叶片和根系中显著表达,可作为美洲狼尾草耐旱性的候选基因,在高温诱导时,Pmccd-6基因在叶片中显著表达,可作为美洲狼尾草耐热性的候选基因,为美洲狼尾草后续的基因工程改良提供帮助.
Abstract
This study aimed to comprehensively characterize the Carotenoids cleave dioxygenases(CCD)gene family in Pennisetum glaucum.Utilizing genome-wide data,identified CCD gene members and predicted their phylogenetic relationships,physicochemical properties,functional domains,and con-served motifs.Additionally,transcriptome data was employed to investigate the expression patterns of PmCCD gene in different tissues under stress conditions.The results showed that a total of 13 PmCCD genes were identified,distributed across five chromosomes.These genes were classified into six subfami-lies within two major categories.All identified PmCCD genes contained either PLN or RPE65 protein domains.Analysis of the promoter region revealed the presence of cis-acting elements associated with stress responses,hormone signaling,and plant growth and development.Transcriptome data exhibited sig-nificantly higher expression of PMccd-1、PMcccd-6、PMccd-7 and PMccd-12 in spike tissue compared to other tissues.Furthermore,PmCCD genes showed significant expression under drought and high tempera-ture stress conditions,with a more pronounced response observed in roots compared to leaves.Notably,Pmccd-12 and Pmccd-6 genes displayed significant expression in leaves and roots under drought stress,suggesting their potential role as candidate genes for drought tolerance in P.glaucum.Similarly,Pmccd-6 gene exhibited significant expression in leaves under high temperature stress,positioning it as a potential candidate gene for heat tolerance.These findings provide valuable insight for future genetic engineering efforts aimed at improving P.glaucum stress tolerance.
关键词
美洲狼尾草/类胡萝卜素裂解双加氧酶/基因家族/干旱和高温胁迫Key words
Pennisetum glaucum/Carotenoid cleavage dioxygenases/Gene family/Drought and heat stress引用本文复制引用
基金项目
四川省饲草育种攻关项目(2021YFYZ0013)
四川省饲草创新团队建设项目(SCCXTD-2020-16)
出版年
2024
NETL
NSTL
万方数据