Genome-wide Identification and Analysis of the Carotenoid Cleavage Dioxygenase(CCD)Gene Family in Pennisetum Glaucum
This study aimed to comprehensively characterize the Carotenoids cleave dioxygenases(CCD)gene family in Pennisetum glaucum.Utilizing genome-wide data,identified CCD gene members and predicted their phylogenetic relationships,physicochemical properties,functional domains,and con-served motifs.Additionally,transcriptome data was employed to investigate the expression patterns of PmCCD gene in different tissues under stress conditions.The results showed that a total of 13 PmCCD genes were identified,distributed across five chromosomes.These genes were classified into six subfami-lies within two major categories.All identified PmCCD genes contained either PLN or RPE65 protein domains.Analysis of the promoter region revealed the presence of cis-acting elements associated with stress responses,hormone signaling,and plant growth and development.Transcriptome data exhibited sig-nificantly higher expression of PMccd-1、PMcccd-6、PMccd-7 and PMccd-12 in spike tissue compared to other tissues.Furthermore,PmCCD genes showed significant expression under drought and high tempera-ture stress conditions,with a more pronounced response observed in roots compared to leaves.Notably,Pmccd-12 and Pmccd-6 genes displayed significant expression in leaves and roots under drought stress,suggesting their potential role as candidate genes for drought tolerance in P.glaucum.Similarly,Pmccd-6 gene exhibited significant expression in leaves under high temperature stress,positioning it as a potential candidate gene for heat tolerance.These findings provide valuable insight for future genetic engineering efforts aimed at improving P.glaucum stress tolerance.
Pennisetum glaucumCarotenoid cleavage dioxygenasesGene familyDrought and heat stress
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