黄花苜蓿MfNAC34基因克隆及特性分析
Cloning and Characteristic Analysis of MfNAC34 in Medicago falcata
张立全 1常娜 2赵志远 1赵永秀1
作者信息
- 1. 内蒙古大学生命科学学院/牧草与特色作物生物学教育部重点实验室,内蒙古 呼和浩特 010070
- 2. 内蒙古大学生命科学学院/牧草与特色作物生物学教育部重点实验室,内蒙古 呼和浩特 010070;呼和浩特市第三十五中学,内蒙古 呼和浩特 010021
- 折叠
摘要
NAC转录因子具有调节植物生长发育和应答非生物胁迫的功能.本研究从内蒙古锡林郭勒草原野生黄花苜蓿材料中克隆获得1个NAC家族蛋白基因MfNAC34(GenBank登录号为MH481768),分析MfNAC34基因编码蛋白的理化性质、亚细胞定位和转录激活特性.结果显示:MfNAC34基因开放阅读框序列为621 bp,编码亲水性蛋白质,分子量为23.52 kD,理论等电点为4.09.MfNAC34为定位在细胞核内的胁迫诱导的转录因子,且转录激活域位于其C端.
Abstract
NAC transcription factor can regulate plant growth and development and respond to abiotic stress.In this study,a NAC family protein gene MfNAC34(GenBank accession number:MH481768)was cloned from Medicago falcata in Xilinguole League steppe of Inner Mongolia,the physicochemical properties,subcellular localization and transcriptional activation activity of MfNAC34 protein were identified.The results showed that the open reding frame sequence of MfNAC34 gene was 621 bp,encoding a hydrophilic protein with a molecular weight of 23.52 kD and a theoretical isoelectric point of 4.09.MfNAC34 was a stress-induced tran-scription factor localized in the nucleus,and the transcription activation domain was located at its C-terminal.
关键词
黄花苜蓿/MfNAC34/特性分析Key words
Medicago falcata/MfNAC34/Characteristic analysis引用本文复制引用
基金项目
国家自然科学基金(31460628)
内蒙古自治区高等学校科学技术研究重点项目(NJZZ22331)
内蒙古自治区应用技术研究与开发项目(2021PT0001)
出版年
2024
NETL
NSTL
万方数据