Whole Genome Identification and Characteristic Analysis of AGO Gene Family in Foxtail Millet
As a highly conserved RNA-induced silencing complex,Argonaute(AGO)proteins are involved in gene transcription and post-transcriptional silencing.At present,the studies on the function of AGO gene in plants mainly focus on Arabidopsis thaliana and rice,but there is no literature report on the function of AGO gene in foxtail millet.In this study,we used bioinformatics methods to identify 15 family members of the foxtail millet with conserved domains and positional sequences on seven chromosomes,it encodes protein length between 862-1148 aa.Phylogenetic analysis showed that these AGO proteins was distributed in three evolutionary branches,and the number of family members was expanded compared with Arabidopsis thaliana.Organizational expression specificity analysis showed that most of the AGO genes in foxtail millet were strongly expressed in panicle,which suggested that they were involved in gene regula-tion of reproductive development.The protein interaction results predicted that all AGO proteins in foxtail millet interact with three proteins,among which DNA-mediated RNA polymerase v subunit 1 has been shown to be associated with gene silencing at the transcription level.These results suggest that the struc-ture and function of AGO gene were conserved during the evolution of foxtail millet,which may provide a theoretical basis for the functional study of AGO gene family members in foxtail millet.
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