34份狼尾草萌发期抗旱性综合评价
Variability in Drought Resistance During Seed Germination among 34 Pennisetum alopecuroides Gerplasms
田宏 1张鹤山 1熊军波 1陆姣云 1吴新江 1刘洋1
作者信息
- 1. 湖北省农业科学院畜牧兽医研究所/湖北省动物胚胎工程及分子育种重点实验室,湖北 武汉 430064
- 折叠
摘要
为评价狼尾草抗旱能力,筛选优异资源,以34份狼尾草材料为对象,研究其PEG-6000胁迫下种子发芽特性,采用隶属函数法和聚类分析对狼尾草抗旱性进行综合评价.结果表明,5%的PEG-6000浓度处理下,狼尾草的发芽率、萌发指数、发芽指数较对照显著增加,表现为促进效应;随着干旱胁迫的增加,各材料发芽受抑作用明显.狼尾草胚芽长和胚根长随PEG浓度的增加呈逐渐下降趋势,其中胚芽较胚根对干旱更敏感.依据测定指标差异程度和变异系数,表明15%的PEG-6000是狼尾草萌发期抗旱性鉴定的适宜浓度.在此浓度处理下,34份狼尾草综合评价值在0.027～0.942之间,其中LW31最高,LW29最低.通过聚类分析所有材料被分为3类,其中LW4、LW9、LW11、LW31、LW42、LW20-2和LW21-1属抗旱材料,可作为狼尾草抗旱性新品种选育的基础材料.
Abstract
To evaluate drought resistance and identify promising germplasm resources,this study inves-tigated the seed germination characteristics of 34 Pennisetum alopecuroides varieties under polyethylene gly-col(PEG-6000)induced drought.Membership function and cluster analysis were employed to comprehen-sively evaluate drought resistance during seed germination.The results showed that compared to the con-trol,5%PEG-6000 concentration exhibited a stimulatory effect,with significantly increased germination rate and index(P<0.05).However,higher PEG-6000 concentration exhibited a progressive inhibitory effect on germination(P<0.05).Plumule length displayed greater sensitivity to drought stress than radicle length,with both decreasing as PEG concentration increased.Fifteen percent of PEG-6000 was determined as suitable for drought resistance evaluation based on the measured parameters.The compre-hensive evaluation index ranged from 0.027 to 0.942 across the 34 varieties,with LW31 exhibiting the highest value and LW29 the lowest.Cluster analysis categorized all varietiesinto three groups.Varieties LW4,LW9,LW11,LW31,LW42,LW20-2,and LW21-1 were classified as drought-tolerant,and hold promise as breeding materials for new drought-resistant P.alopecuroides varieties.
关键词
狼尾草/干旱胁迫/种子萌发/鉴定浓度/综合评价Key words
Pennisetum alopecuroides/Drought stress/Seed germination/Identification concentra-tion/Comprehensive evaluation引用本文复制引用
基金项目
国家牧草产业技术体系建设项目(CARS-34)
湖北省农业科技创新中心项目(2021-620-000-001-021)
出版年
2024
NETL
NSTL
万方数据